The understanding of human diseases has been greatly expanded thanks to the study of animal models. Nonetheless, histopathological evaluation of experimental models needs to be as rigorous as that applied for human samples. Indeed, drawing reliable and accurate conclusions is critically influenced by the quality of tissue section preparation. Here, we describe a protocol for histopathological analysis of murine tissues that implements several automated steps during the procedure, from the initial preparation to the paraffin embedding of the murine samples. The reduction of methodological variables through rigorous protocol standardization from automated procedures contributes to increased overall reliability of murine pathological analysis. Specifically, this protocol describes the utilization of automated processing and embedding robotic systems, routinely used for the tissue processing and paraffin embedding of human samples, to process murine specimens of intestinal inflammation. We conclude that the reliability of histopathological examination of murine tissues is significantly increased upon introduction of standardized and automated techniques.

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses / F.M. Cribiù, C. Burrello, R. Tacchi, F. Boggio, D. Ricca, F. Caprioli, S. Ferrero, F. Facciotti. - In: JOURNAL OF VISUALIZED EXPERIMENTS. - ISSN 1940-087X. - 2019:143(2019 Jan 07). [10.3791/58654]

Using Robotic Systems to Process and Embed Colonic Murine Samples for Histological Analyses

C. Burrello;F. Boggio;F. Caprioli;S. Ferrero;
2019

Abstract

The understanding of human diseases has been greatly expanded thanks to the study of animal models. Nonetheless, histopathological evaluation of experimental models needs to be as rigorous as that applied for human samples. Indeed, drawing reliable and accurate conclusions is critically influenced by the quality of tissue section preparation. Here, we describe a protocol for histopathological analysis of murine tissues that implements several automated steps during the procedure, from the initial preparation to the paraffin embedding of the murine samples. The reduction of methodological variables through rigorous protocol standardization from automated procedures contributes to increased overall reliability of murine pathological analysis. Specifically, this protocol describes the utilization of automated processing and embedding robotic systems, routinely used for the tissue processing and paraffin embedding of human samples, to process murine specimens of intestinal inflammation. We conclude that the reliability of histopathological examination of murine tissues is significantly increased upon introduction of standardized and automated techniques.
Neuroscience (all); Chemical Engineering (all); Biochemistry, Genetics and Molecular Biology (all); Immunology and Microbiology (all); Immunology and Infection; Issue 143; histopathological analysis; murine models; automation; embedding system; standardization; murine intestinal specimens
Settore MED/04 - Patologia Generale
Settore MED/12 - Gastroenterologia
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/616172
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