Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis.

TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro / S. Castiglioni, V. Romeo, L. Locatelli, A. Cazzaniga, J.A.M. Maier. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 8:1(2018 Nov 01). [10.1038/s41598-018-34324-8]

TRPM7 and MagT1 in the osteogenic differentiation of human mesenchymal stem cells in vitro

S. Castiglioni
Primo
;
V. Romeo
Secondo
;
L. Locatelli;A. Cazzaniga
Penultimo
;
J.A.M. Maier
Ultimo
2018

Abstract

Mesenchymal stem cells are fundamental for bone formation and repair since they respond to microenvironmental stimuli by undergoing osteogenic differentiation. We show that the kinase and cation channel TRPM7 and the magnesium transporter MagT1 have a role in harmonizing the osteogenic differentiation of human mesenchymal stem cells. TRPM7 and MagT1 are upregulated in osteogenic differentiation and silencing either one accelerates osteogenic differentiation, partly through the activation of autophagy. Intriguingly, similar results were obtained when the cells were cultured under magnesium deficient conditions. These results underpin the contribution of magnesium, TRPM7 and MagT1 to autophagy and osteoblastogenesis.
Settore MED/04 - Patologia Generale
1-nov-2018
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/614694
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