Introduction: Chronic spontaneous urticaria (CSU) is characterized by recurrent itchy wheals, angioedema or both, that persist for longer than six weeks. In children, up to 40% of chronic spontaneous urticaria is due to mast cells and basophils-activating autoantibodies, mostly directed against the IgE high-affinity receptor subunit (FcεRI). Indirect basophil activation test (BAT) has been proposed in the diagnosis of autoimmune urticaria. Materials and methods: Sera from sixteen patients, aged from 2 to 15 yrs, with CSU were evaluated through indirect BAT by flow cytometry using a commercial kit (Flow CAST®, BUHLMANN Laboratories, Schonenbuch, Switzerland) according to the manufacturer's instructions. Results: Indirect BAT test gave a much better diagnosis in our cohort than the gold standard ASST. Six children (37.5%) showed a positive indirect BAT while we could perform ASST in only 3 patients with just one patients showing a positive ASST. The specificity of BAT positive results was confirmed by the absence of significant difference between the BAT results obtained from negative controls vs negative sera of the patient (p = 0.65) on the basophil donors, indicating that the serum is not activating basophil per se. Conclusions: This pilot study suggests the utility of BAT to identify the subtype of autoimmune CSU in children in clinical practice.

Basophil activation test in children with autoimmune chronic spontaneous urticaria: Is it ready for clinical practice? / E. D'Auria, M. De Amici, A. Licari, S. Caimmi, C. Mantegazza, G.V. Zuccotti, G. Marseglia. - In: IMMUNOBIOLOGY. - ISSN 0171-2985. - 224:1(2019 Jan), pp. 30-33. [10.1016/j.imbio.2018.11.001]

Basophil activation test in children with autoimmune chronic spontaneous urticaria: Is it ready for clinical practice?

E. D'Auria;G.V. Zuccotti;
2019

Abstract

Introduction: Chronic spontaneous urticaria (CSU) is characterized by recurrent itchy wheals, angioedema or both, that persist for longer than six weeks. In children, up to 40% of chronic spontaneous urticaria is due to mast cells and basophils-activating autoantibodies, mostly directed against the IgE high-affinity receptor subunit (FcεRI). Indirect basophil activation test (BAT) has been proposed in the diagnosis of autoimmune urticaria. Materials and methods: Sera from sixteen patients, aged from 2 to 15 yrs, with CSU were evaluated through indirect BAT by flow cytometry using a commercial kit (Flow CAST®, BUHLMANN Laboratories, Schonenbuch, Switzerland) according to the manufacturer's instructions. Results: Indirect BAT test gave a much better diagnosis in our cohort than the gold standard ASST. Six children (37.5%) showed a positive indirect BAT while we could perform ASST in only 3 patients with just one patients showing a positive ASST. The specificity of BAT positive results was confirmed by the absence of significant difference between the BAT results obtained from negative controls vs negative sera of the patient (p = 0.65) on the basophil donors, indicating that the serum is not activating basophil per se. Conclusions: This pilot study suggests the utility of BAT to identify the subtype of autoimmune CSU in children in clinical practice.
Autoimmune chronic urticaria; Autologous serum skin test; Basophil activation test; Chronic spontaneous urticaria; IgE high-affinity receptor subunit; Immunology and Allergy; Immunology; Hematology
Settore MED/38 - Pediatria Generale e Specialistica
2018
Article (author)
File in questo prodotto:
File Dimensione Formato  
Immunobiology. 2018.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 179.08 kB
Formato Adobe PDF
179.08 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/614279
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 4
  • ???jsp.display-item.citation.isi??? 4
social impact