Fused Filament Fabrication (FFF) three-dimensional printing have attracted much attention for fabrication of microfluidic platforms used to construct electrochemical microfluidic biosensors because of high process speed, low production costs and the possibility of manufacturing directly from virtual data. Because of poor adhesion between metal electrodes fabricated using conventional techniques and FFF printed thermoplastic substrates, electrodes are usually integrated into the devices either modularly or using adhesive layers placed at the bottom of fluidic channels. These have hindered the exploitation of FFF for scale-up manufacturing of monolithically integrated microfluidic biosensors. In this work, supersonic cluster beam deposition (SCBD) was employed to fabricate strongly anchored nanostructured electrodes integrated into FFF printed microfluidics platforms. SCBD enables the formation of well-adhering metallic thin film electrodes by implanting supersonically accelerated neutral metal clusters into polymeric substrates. The SCBD also enables deposition over large areas using noble metals and metal oxides with precisely controlled geometry and surface topography. A novel integrated manufacturing approach was developed and optimized to couple SCBD fabricated electrodes with consumer-grade FFF printed microfluidics, employing acrylonitrile butadiene styrene as the base material, to develop a three electrodes configuration electrochemical sensor on-a-chip. Electrochemical investigation performed using stagnant ferro/ferricyanide probe showed that the integrated device possesses high sensitivity and functionality as an electrochemical sensor. In addition, in-channel laminar flow electrochemical detection conducted using the same probe showed robust stability in the system response for online dynamic detection. The integrated platform could be employed for various customized clinical, industrial, and environmental sensing applications.

AN ELECTROCHEMICAL MICROFLUIDIC BIOSENSOR PLATFORM FABRICATED BY ADDITIVE MANUFACTURING AND SUPERSONIC CLUSTER BEAM DEPOSITION / W.a. Gebreyes ; tutor: Paolo Milani. DIPARTIMENTO DI FISICA, 2019 Jan 28. 30. ciclo, Anno Accademico 2018. [10.13130/gebreyes-wondimu-alemu_phd2019-01-28].

AN ELECTROCHEMICAL MICROFLUIDIC BIOSENSOR PLATFORM FABRICATED BY ADDITIVE MANUFACTURING AND SUPERSONIC CLUSTER BEAM DEPOSITION

W.A. Gebreyes
2019

Abstract

Fused Filament Fabrication (FFF) three-dimensional printing have attracted much attention for fabrication of microfluidic platforms used to construct electrochemical microfluidic biosensors because of high process speed, low production costs and the possibility of manufacturing directly from virtual data. Because of poor adhesion between metal electrodes fabricated using conventional techniques and FFF printed thermoplastic substrates, electrodes are usually integrated into the devices either modularly or using adhesive layers placed at the bottom of fluidic channels. These have hindered the exploitation of FFF for scale-up manufacturing of monolithically integrated microfluidic biosensors. In this work, supersonic cluster beam deposition (SCBD) was employed to fabricate strongly anchored nanostructured electrodes integrated into FFF printed microfluidics platforms. SCBD enables the formation of well-adhering metallic thin film electrodes by implanting supersonically accelerated neutral metal clusters into polymeric substrates. The SCBD also enables deposition over large areas using noble metals and metal oxides with precisely controlled geometry and surface topography. A novel integrated manufacturing approach was developed and optimized to couple SCBD fabricated electrodes with consumer-grade FFF printed microfluidics, employing acrylonitrile butadiene styrene as the base material, to develop a three electrodes configuration electrochemical sensor on-a-chip. Electrochemical investigation performed using stagnant ferro/ferricyanide probe showed that the integrated device possesses high sensitivity and functionality as an electrochemical sensor. In addition, in-channel laminar flow electrochemical detection conducted using the same probe showed robust stability in the system response for online dynamic detection. The integrated platform could be employed for various customized clinical, industrial, and environmental sensing applications.
28-gen-2019
Settore FIS/03 - Fisica della Materia
Centro Interdisciplinare Materiali ed Interfacce Nanostrutturati - CIMAINA
Microfluids, biosensors, Fused Filament Fabrication
MILANI, PAOLO
Doctoral Thesis
AN ELECTROCHEMICAL MICROFLUIDIC BIOSENSOR PLATFORM FABRICATED BY ADDITIVE MANUFACTURING AND SUPERSONIC CLUSTER BEAM DEPOSITION / W.a. Gebreyes ; tutor: Paolo Milani. DIPARTIMENTO DI FISICA, 2019 Jan 28. 30. ciclo, Anno Accademico 2018. [10.13130/gebreyes-wondimu-alemu_phd2019-01-28].
File in questo prodotto:
File Dimensione Formato  
phd_unimi_R11156.pdf

Open Access dal 19/07/2020

Descrizione: tesi di dottorato
Tipologia: Tesi di dottorato completa
Dimensione 7.09 MB
Formato Adobe PDF
7.09 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/613065
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact