The complete gene (amy) that encoding a raw starch digesting α amylase (BYXA) from Bacillus sp. YX-1 was cloned, sequenced and expressed in Escherichia coli. The amy gene consisted of 1545 base pairs and encoded a protein of 514 amino acid residues with a calculated molecular mass of 58 kDa. The recombinant protein was purified by Ni2+ affinity chromatography and subsequently characterized. The optimal pH and temperature for the purified recombinant enzyme were 5.5 and 45 °C, respectively. The enzyme showed stability over a pH range of 5.0-11.0 and below 50 °C. The enzyme exhibited remarkable digestibility towards raw starch at low pH values, which was valuable in starch processing industry.
Effect of indigenous bacterial community on arsenic mobilization in a degraded soil under submerged conditions / A. Corsini, L. Cavalca, L. Crippa, P. Zaccheo, V. Andreoni. - In: ANNALS OF MICROBIOLOGY. - ISSN 1590-4261. - 59:Special issue(2009), pp. 91-91. ((Intervento presentato al 2. convegno National Conference SIMTREA tenutosi a Sassari nel 2009.
|Titolo:||Effect of indigenous bacterial community on arsenic mobilization in a degraded soil under submerged conditions|
CORSINI, ANNA (Primo)
CAVALCA, LUCIA (Secondo)
ZACCHEO, PATRIZIA (Penultimo)
ANDREONI, VINCENZINA (Ultimo)
|Settore Scientifico Disciplinare:||Settore AGR/13 - Chimica Agraria|
Settore AGR/16 - Microbiologia Agraria
|Data di pubblicazione:||2009|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/BF03175604|
|Appare nelle tipologie:||01 - Articolo su periodico|