Fruits protect the developing seeds of angiosperms and actively contribute to seed dispersion. Furthermore, fruit and seed development are highly synchronized and require exchange of information between the mother plant and the developing generations. To explore the mechanisms controlling fruit formation and maturation, we performed a transcriptomic analysis on the valve tissue of the Arabidopsis (Arabidopsis thaliana) silique using RNAseq. In doing so, we have generated a dataset of differentially regulated genes that will help to elucidate the molecular mechanisms that underpin the initial phase of fruit growth, and subsequently trigger fruit maturation. The robustness of our dataset has been tested by functional genomic studies. Using a reverse genetics approach, we selected 10 differentially expressed genes and explored the consequences of their disruption for both silique growth and senescence. We found that genes contained in our dataset play essential roles in different stages of silique development and maturation, indicating that our transcriptome-based gene list is a powerful tool for the elucidation of the molecular mechanisms controlling fruit formation in Arabidopsis.

Time-course transcriptome analysis of Arabidopsis siliques discloses genes essential for fruit development and maturation / C. Mizzotti, L. Rotasperti, M. Moretto, L. Tadini, F. Resentini, B. Galliani, M. Galbiati, K. Engelen, P. Pesaresi, S. Masiero. - In: PLANT PHYSIOLOGY. - ISSN 0032-0889. - 178:3(2018 Nov), pp. 1249-1268. [10.1104/pp.18.00727]

Time-course transcriptome analysis of Arabidopsis siliques discloses genes essential for fruit development and maturation

C. Mizzotti;L. Rotasperti;L. Tadini;F. Resentini;B. Galliani;M. Galbiati;P. Pesaresi;S. Masiero
2018

Abstract

Fruits protect the developing seeds of angiosperms and actively contribute to seed dispersion. Furthermore, fruit and seed development are highly synchronized and require exchange of information between the mother plant and the developing generations. To explore the mechanisms controlling fruit formation and maturation, we performed a transcriptomic analysis on the valve tissue of the Arabidopsis (Arabidopsis thaliana) silique using RNAseq. In doing so, we have generated a dataset of differentially regulated genes that will help to elucidate the molecular mechanisms that underpin the initial phase of fruit growth, and subsequently trigger fruit maturation. The robustness of our dataset has been tested by functional genomic studies. Using a reverse genetics approach, we selected 10 differentially expressed genes and explored the consequences of their disruption for both silique growth and senescence. We found that genes contained in our dataset play essential roles in different stages of silique development and maturation, indicating that our transcriptome-based gene list is a powerful tool for the elucidation of the molecular mechanisms controlling fruit formation in Arabidopsis.
development; fruit development; organelles - biogenesis/development; senescence; fruit Ripening
Settore BIO/01 - Botanica Generale
Settore BIO/18 - Genetica
Settore BIO/04 - Fisiologia Vegetale
   Towards the comprehension of the seed-fruit crosstalk
   SeeFruit
   FONDAZIONE CARIPLO
   2011-2257

   Investigating Self Incompatibility DEterminants in fruit trees (ISIDE)
   MINISTERO DELL'ISTRUZIONE E DEL MERITO
   2015BPM9H3_005
nov-2018
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/612702
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