The recently discovered globin-coupled sensors (GCS) are heme-containing two domain transducers, that mediate the cellular responses to metabolic and environmental stimuli, such as NO, CO and O2. In particular, the GCSs identified in the strict anaerobic -Proteobacteria Geobacter sulfurreducens and Geobacter metalloreducens may be involved in sulfate/sulfur reduction. They are constituted by 300 amino acid residues and characterised by a N-terminal globin domain (162 amino acids) and by a bundle of four transmembrane helices at C-terminal . Here, we present the X-ray three-dimensional structure of the globin domain of GCS from Geobacter sulfurreducens (Gs-GCS162), at 1.5 Å resolution. The crystal structure of ferric Gs-GCS162 was solved by MAD techniques (data collected at the synchrotron beamline ID23-1, ESRF, Grenoble, France), based on the heme Fe atom anomalous scattering. The Gs-GCS162 fold is a variant of the classical globin fold of mioglobin and hemoglobin, with helices A through H, and an additional Z helix at the N-terminus. The Gs-GCS162 displays a six-coordinated heme and is one of the first example of bishistidyl hexacoordinated GCS. Strikingly, the distal heme-coordination is provided by a His residue at topological position E11, an unprecedented feature within globins.
|Titolo:||The three-dimensional structure of the globin domain of globin-coupled sensor from Geobacter sulfurreducens|
|Autori interni:||NARDINI, MARCO (Secondo)|
BOLOGNESI, MARTINO (Ultimo)
|Data di pubblicazione:||ago-2008|
|Parole Chiave:||globin-coupled sensor ; heme protein|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Citazione:||The three-dimensional structure of the globin domain of globin-coupled sensor from Geobacter sulfurreducens / A. Pesce, M. Nardini, L. Thijs, S. Dewilde, A. Penco, P. Ascenzi, L. Moens, M. Bolognesi. ((Intervento presentato al 15. convegno International Conference on Oxygen Binding and Sensing Proteins tenutosi a Århus (Danimarca) nel 2008.|
|Appare nelle tipologie:||14 - Intervento a convegno non pubblicato|
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