We compared 3 major cross-match (XM) tests to identify dog erythrocyte antigen (DEA) 7 blood incompatibilities in dogs as a result of anti–DEA 7 antibodies: gel (GEL), standard tube (TUBE) agglutination, and immunochromatography strips (STRIP). Blood samples from 42 dogs were typed for DEA 7; 2 tested DEA 7–positive (DEA 7+). The 40 DEA 7–negative (DEA 7–) plasma samples were cross-matched against the 2 DEA 7+ and 3 DEA 7– red blood cell (RBC) samples by GEL to identify samples with anti–DEA 7 antibodies. Twenty DEA 7– plasma samples without and with anti–DEA 7 antibodies were cross-matched with samples of the 2 DEA 7+ RBCs in a double-blind fashion using the TUBE and STRIP XM methods. GEL results were used as the reference method for comparison. To determine relationships between results, 2 × 2 tables were used. Cohen kappa coefficient (κ) was calculated between results of GEL and the other 2 methods. With GEL, 21 of 40 XM tests were positive and 19 of 40 negative for anti–DEA 7 antibodies. The same results were obtained by TUBE, whereas only 1 of 40 XM tests was positive by STRIP. There was a statistically significant relationship between results of GEL and TUBE (p < 0.000) with perfect agreement (κ = 1.000), but not between GEL and STRIP results (p = 1.000) in which agreement was equivalent to chance (κ = 0.0453). The GEL and TUBE XM tests, but not STRIP, are useful methods for identification of DEA 7 incompatibilities caused by anti–DEA 7 antibodies.
Comparison of cross-matching method for detection of DEA 7 blood incompatibility / E. Spada, R. Perego, L. Miguel Viñals Flórez, M. del Rosario Perlado Chamizo, L. Baggiani, D. Paola, D. Proverbio. - In: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. - ISSN 1040-6387. - 30:6(2018 Nov), pp. 911-916.
|Titolo:||Comparison of cross-matching method for detection of DEA 7 blood incompatibility|
SPADA, EVA (Corresponding)
|Parole Chiave:||Anti–DEA 7 antibodies; blood typing; canine; cross-matching; gel; immunochromatography; tube|
|Settore Scientifico Disciplinare:||Settore VET/08 - Clinica Medica Veterinaria|
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
|Data di pubblicazione:||nov-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1177/1040638718806023|
|Appare nelle tipologie:||01 - Articolo su periodico|
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