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EnglishWe compared 3 major cross-match (XM) tests to identify dog erythrocyte antigen (DEA) 7 blood incompatibilities in dogs as a result of anti–DEA 7 antibodies: gel (GEL), standard tube (TUBE) agglutination, and immunochromatography strips (STRIP). Blood samples from 42 dogs were typed for DEA 7; 2 tested DEA 7–positive (DEA 7+). The 40 DEA 7–negative (DEA 7–) plasma samples were cross-matched against the 2 DEA 7+ and 3 DEA 7– red blood cell (RBC) samples by GEL to identify samples with anti–DEA 7 antibodies. Twenty DEA 7– plasma samples without and with anti–DEA 7 antibodies were cross-matched with samples of the 2 DEA 7+ RBCs in a double-blind fashion using the TUBE and STRIP XM methods. GEL results were used as the reference method for comparison. To determine relationships between results, 2 × 2 tables were used. Cohen kappa coefficient (κ) was calculated between results of GEL and the other 2 methods. With GEL, 21 of 40 XM tests were positive and 19 of 40 negative for anti–DEA 7 antibodies. The same results were obtained by TUBE, whereas only 1 of 40 XM tests was positive by STRIP. There was a statistically significant relationship between results of GEL and TUBE (p < 0.000) with perfect agreement (κ = 1.000), but not between GEL and STRIP results (p = 1.000) in which agreement was equivalent to chance (κ = 0.0453). The GEL and TUBE XM tests, but not STRIP, are useful methods for identification of DEA 7 incompatibilities caused by anti–DEA 7 antibodies.
Comparison of cross-matching method for detection of DEA 7 blood incompatibility / E. Spada, R. Perego, L. Miguel Viñals Flórez, M. del Rosario Perlado Chamizo, L. Baggiani, D. Paola, D. Proverbio. - In: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. - ISSN 1040-6387. - 30:6(2018 Nov), pp. 911-916.
| Titolo: | Comparison of cross-matching method for detection of DEA 7 blood incompatibility |
| Autori: | |
| Parole Chiave: | Anti–DEA 7 antibodies; blood typing; canine; cross-matching; gel; immunochromatography; tube |
| Settore Scientifico Disciplinare: | Settore VET/08 - Clinica Medica Veterinaria Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria |
| Data di pubblicazione: | nov-2018 |
| Rivista: | |
| Tipologia: | Article (author) |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1177/1040638718806023 |
| Appare nelle tipologie: | 01 - Articolo su periodico |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| per irisSPADA17-0476.R3-pk-ANTI-DEA7-mgm2018.06.23-hf2018.09.17mgm.doc | Pre-print (manoscritto inviato all'editore) | Open Access Visualizza/Apri | ||
| 1040638718806023.pdf | Publisher's version/PDF | Open Access Visualizza/Apri |
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