The formation of intramolecular contacts between distant residues is, perhaps, the simplest among the elementary processes taking place during the folding of a protein. Quenching of the triplet state of tryptophan by close contact with cysteine after nanosecond laser excitation has been used to measure both equilibrium (reaction-limited) and dynamic (diffusion-limited) contact formation rates in model peptides and engineered proteins. The triplet quenching data and FRET end-to-end distances are analyzed by means of a chain model with realistic backbone conformations and simplified hard-sphere interaction between the residues. The effect of amino acid sequence on the chain swelling is discussed.

Kinetics of intramolecular contact formation in disordered peptides and unfolded proteins / A. Soranno, T. Bellini, M. Buscaglia. ((Intervento presentato al 94. convegno Congresso Nazionale della Società Italiana di Fisica tenutosi a Genova nel 2008.

Kinetics of intramolecular contact formation in disordered peptides and unfolded proteins

A. Soranno
Primo
;
T. Bellini
Secondo
;
M. Buscaglia
Ultimo
2008-09

Abstract

The formation of intramolecular contacts between distant residues is, perhaps, the simplest among the elementary processes taking place during the folding of a protein. Quenching of the triplet state of tryptophan by close contact with cysteine after nanosecond laser excitation has been used to measure both equilibrium (reaction-limited) and dynamic (diffusion-limited) contact formation rates in model peptides and engineered proteins. The triplet quenching data and FRET end-to-end distances are analyzed by means of a chain model with realistic backbone conformations and simplified hard-sphere interaction between the residues. The effect of amino acid sequence on the chain swelling is discussed.
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
Società Italiana di Fisica (SIF)
Kinetics of intramolecular contact formation in disordered peptides and unfolded proteins / A. Soranno, T. Bellini, M. Buscaglia. ((Intervento presentato al 94. convegno Congresso Nazionale della Società Italiana di Fisica tenutosi a Genova nel 2008.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/59281
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