The efficiency of in vitro assisted reproductive technologies, consisting of the transfer of embryos obtained in vitro through in vitro maturation, in vitro fertilization and early embryo culture is still limited. The quality of the oocytes is pivotal for assisted reproductive efficiency and the maturation of the oocyte represents the first key limiting step of the in vitro embryo production system. At the time of removal from the antral follicles, the oocyte is still completing the final growth and differentiation steps, needed to provide the so-called developmental competence, i.e. the machinery required to sustain fertilization and embryo development. In mono-ovular species only one oocyte per cycle is available for procreation, therefore the current assisted reproduction techniques strive to overcome this natural boundary. However, the success is still limited and overall the effectiveness does not exceed the efficiency achieved in millions of years of mammalian evolution. One of the problems lies in the intrinsic heterogeneity of the oocytes that are subjected to in vitro maturation and in the lack of dedicated in vitro approaches to finalize the differentiation process. In this review we will try to overview some of the salient aspects of current practices by emphasizing the most critical and fundamental features in oocyte differentiation that should be carefully considered for improving current techniques.

The variable success of in vitro maturation: can we do better? / A.M. Luciano, F. Franciosi, R. Garcia Barros, C. Dieci, V. Lodde. - In: ANIMAL REPRODUCTION. - ISSN 1806-9614. - 15:suppl. 1(2018), pp. 727-736. ((Intervento presentato al 10. convegno International Ruminant Reproduction Symposium tenutosi a Foz do Iguaçu nel 2018 [10.21451/1984-3143-AR2018-0021].

The variable success of in vitro maturation: can we do better?

A.M. Luciano
Writing – Original Draft Preparation
;
F. Franciosi
Conceptualization
;
R. Garcia Barros
Membro del Collaboration Group
;
C. Dieci
Methodology
;
V. Lodde
Writing – Original Draft Preparation
2018

Abstract

The efficiency of in vitro assisted reproductive technologies, consisting of the transfer of embryos obtained in vitro through in vitro maturation, in vitro fertilization and early embryo culture is still limited. The quality of the oocytes is pivotal for assisted reproductive efficiency and the maturation of the oocyte represents the first key limiting step of the in vitro embryo production system. At the time of removal from the antral follicles, the oocyte is still completing the final growth and differentiation steps, needed to provide the so-called developmental competence, i.e. the machinery required to sustain fertilization and embryo development. In mono-ovular species only one oocyte per cycle is available for procreation, therefore the current assisted reproduction techniques strive to overcome this natural boundary. However, the success is still limited and overall the effectiveness does not exceed the efficiency achieved in millions of years of mammalian evolution. One of the problems lies in the intrinsic heterogeneity of the oocytes that are subjected to in vitro maturation and in the lack of dedicated in vitro approaches to finalize the differentiation process. In this review we will try to overview some of the salient aspects of current practices by emphasizing the most critical and fundamental features in oocyte differentiation that should be carefully considered for improving current techniques.
oocyte; in vitro maturation; development
Settore VET/01 - Anatomia degli Animali Domestici
Settore VET/02 - Fisiologia Veterinaria
Settore BIO/06 - Anatomia Comparata e Citologia
Settore BIO/09 - Fisiologia
Settore BIO/11 - Biologia Molecolare
Settore BIO/17 - Istologia
2018
CBRA; SBTE; CAPES; CNPq; SRF; SSR
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/591706
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