We devised and fabricated a chemosensor for determination of the genetically relevant 5′-GCGGCGGC-3′ (G = guanine; C = cytosine) oligonucleotide. For that, we simultaneously electrosynthesized and electrode-immobilized a sequence-defined octakis(2,2′-bithien-5-yl) DNA hybridizing probe using both a "macromolecular imprinting in polymer strategy" and a sequence-programmable peptide nucleic acid (PNA) template. With electrochemical impedance spectroscopy (EIS) and surface plasmon resonance (SPR) transductions under stagnant-solution and flow injection analysis (FIA) conditions, respectively, we determined the above oligonucleotide with 200-pM EIS limit of detection. With its EIS-determined apparent imprinting factor of ∼4.0, the chemosensor was discriminative to both mismatched oligonucleotides and Dulbecco's modified Eagle's medium sample interferences.
Oligonucleotide Determination via Peptide Nucleic Acid Macromolecular Imprinting in an Electropolymerized CG-Rich Artificial Oligomer Analogue / K. Bartold, A. Pietrzyk-Le, K. Golebiewska, W. Lisowski, S. Cauteruccio, E. Licandro, F. D'Souza, W. Kutner. - In: ACS APPLIED MATERIALS & INTERFACES. - ISSN 1944-8244. - 10:33(2018), pp. 27562-27569.
Titolo: | Oligonucleotide Determination via Peptide Nucleic Acid Macromolecular Imprinting in an Electropolymerized CG-Rich Artificial Oligomer Analogue | |
Autori: | ||
Parole Chiave: | CG-rich DNA analogue; DNA sensor; electrochemical impedance spectroscopy; hybridization; isothermal titration calorimetry; macromolecularly imprinted polymer; peptide nucleic acid; Materials Science (all) | |
Settore Scientifico Disciplinare: | Settore CHIM/06 - Chimica Organica | |
Data di pubblicazione: | 2018 | |
Rivista: | ||
Tipologia: | Article (author) | |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1021/acsami.8b09296 | |
Appare nelle tipologie: | 01 - Articolo su periodico |
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