Bisphenol A (BPA) is an organic pollutant derived from plastic degradation that has numerous and variable adverse effects on human health and wildlife. In particular, it has been reported that BPA can alter reproductive processes and nervous system development in vertebrates. Considering BPA presence in marine environment and the scant data available on its interaction with nervous system development, we analyzed the effect of BPA exposure on sperm viability, fertilization, embryogenesis, and neural differentiation of the ascidian Ciona robusta. Ascidians are members of the Phylum Tunicata, the sister group of Vertebrata, sharing with them fundamental developmental processes. Our results showed that first cell division was altered starting from 5 mu M concentration. Lethal concentration (LC50) was estimated to be 5.2 mu M. Larvae developed from treated embryos showed specific malformations to the pigment cells even at 0.1 mu M, corresponding to the highest environmental concentration reported so far. Moreover, GABAergic and dopaminergic neurons proved to be target organs of BPA teratogenic action, in accordance with similar results reported in vertebrate animal models. Overall, our results suggest that BPA can exert its effects on nervous system acting on different pathways and underline that C. robusta is a valuable invertebrate animal model for preliminary screenings of effects of pollutants on vertebrates.

Bisphenol A affects neural development of the ascidian Ciona robusta / S. Messinetti, S. Mercurio, R. Pennati. - In: JOURNAL OF EXPERIMENTAL ZOOLOGY. PART A, ECOLOGICAL GENETICS AND PHYSIOLOGY. - ISSN 1932-5223. - 331:1(2019 Jan 01), pp. 5-16.

Bisphenol A affects neural development of the ascidian Ciona robusta

S. Messinetti
Primo
;
S. Mercurio
Secondo
;
R. Pennati
Ultimo
2019

Abstract

Bisphenol A (BPA) is an organic pollutant derived from plastic degradation that has numerous and variable adverse effects on human health and wildlife. In particular, it has been reported that BPA can alter reproductive processes and nervous system development in vertebrates. Considering BPA presence in marine environment and the scant data available on its interaction with nervous system development, we analyzed the effect of BPA exposure on sperm viability, fertilization, embryogenesis, and neural differentiation of the ascidian Ciona robusta. Ascidians are members of the Phylum Tunicata, the sister group of Vertebrata, sharing with them fundamental developmental processes. Our results showed that first cell division was altered starting from 5 mu M concentration. Lethal concentration (LC50) was estimated to be 5.2 mu M. Larvae developed from treated embryos showed specific malformations to the pigment cells even at 0.1 mu M, corresponding to the highest environmental concentration reported so far. Moreover, GABAergic and dopaminergic neurons proved to be target organs of BPA teratogenic action, in accordance with similar results reported in vertebrate animal models. Overall, our results suggest that BPA can exert its effects on nervous system acting on different pathways and underline that C. robusta is a valuable invertebrate animal model for preliminary screenings of effects of pollutants on vertebrates.
dopamine; embryogenesis; environmental pollutant; GABA; pigmented organs
Settore BIO/05 - Zoologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/589925
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