Objective[s]: Activin A and inhibin A are growth factors expressed by human endometrium involved in the control of endometrial functions. In the present study we investigated the effects of activin A and inhibin A in modulating the tumor necrosis factor (TNF)-a/intercellular adhesion molecule (ICAM)-1 system in cultured human endometrial stromal cells. Study design: Endometrial samples were obtained from 34 reproductive age women undergoing laparoscopy for benign ovarian cysts or infertility. Endometrial stromal cells were cultured and soluble ICAM-1 and TNF-a were measured in cell-free supernatants following treatment with or without activin A or inhibin A. Cell surface ICAM-1 was assayed by flow cytometry by staining endometrial cells with specific monoclonal antibodies. Results: Activin A and inhibin A did not influence either the expression of cell surface ICAM-1 or soluble ICAM-1 shedding by cultured endometrial cells. On the other hand, TNF-a secretion significantly increased in presence of activin A but not of inhibin A. Conclusions: Since TNF-a modulates several endometrial processes such as menstruation, proliferation, apoptosis, implantation and decidualization, an effect of activin A in the physiological control of endometrium is further supported by the present data.
|Titolo:||Effect of activin A on tumor necrosis factor-alpha/intercellular adhesion molecule-1 pathway in endometrial stromal cells|
|Autori interni:||VIGNALI, MICHELE|
MANGIONI, SILVIA (Primo)
VIGANO', PAOLA (Secondo)
|Parole Chiave:||Activin A; Endometrium; ICAM-1; TNF-α|
|Settore Scientifico Disciplinare:||Settore MED/40 - Ginecologia e Ostetricia|
|Data di pubblicazione:||dic-2005|
|Digital Object Identifier (DOI):||10.1016/j.ejogrb.2005.03.017|
|Appare nelle tipologie:||01 - Articolo su periodico|
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