Iron recycling by macrophages is essential for erythropoiesis, but may be also relevant for iron redistribution to neighbouring cells at the local tissue level. Using mice with iron retention in macrophages due to targeted inactivation of the iron exporter ferroportin, we investigated the role of macrophage iron release in hair follicle cycling and wound healing, a complex process leading to major clinical problems, if impaired. Genetic deletion of ferroportin in macrophages resulted in iron deficiency and decreased proliferation in epithelial cells, which consequently impaired hair follicle growth and caused transient alopecia. Hair loss was not related to systemic iron deficiency or anemia, thus indicating the necessity of local iron release from macrophages. Inactivation of macrophage ferroportin also led to delayed skin wound healing with defective granulation tissue formation and diminished fibroplasia. Iron retention in macrophages had no impact on the inflammatory processes accompanying wound healing, but affected stromal cells proliferation, blood and lymphatic vessels formation, and fibrogenesis. Our findings reveal that iron/ferroportin plays a largely underestimated role in the macrophage trophic function in skin homeostasis and repair.

Macrophage ferroportin is essential for stromal cell proliferation in wound healing / S. Recalcati, E. Gammella, P. Buratti, A. Doni, A. Anselmo, M. Locati, G. Cairo. - In: HAEMATOLOGICA. - ISSN 0390-6078. - 104:1(2019 Jan), pp. 47-58.

Macrophage ferroportin is essential for stromal cell proliferation in wound healing

S. Recalcati
Co-primo
;
E. Gammella
Co-primo
;
P. Buratti;M. Locati
Penultimo
;
G. Cairo
Ultimo
2019

Abstract

Iron recycling by macrophages is essential for erythropoiesis, but may be also relevant for iron redistribution to neighbouring cells at the local tissue level. Using mice with iron retention in macrophages due to targeted inactivation of the iron exporter ferroportin, we investigated the role of macrophage iron release in hair follicle cycling and wound healing, a complex process leading to major clinical problems, if impaired. Genetic deletion of ferroportin in macrophages resulted in iron deficiency and decreased proliferation in epithelial cells, which consequently impaired hair follicle growth and caused transient alopecia. Hair loss was not related to systemic iron deficiency or anemia, thus indicating the necessity of local iron release from macrophages. Inactivation of macrophage ferroportin also led to delayed skin wound healing with defective granulation tissue formation and diminished fibroplasia. Iron retention in macrophages had no impact on the inflammatory processes accompanying wound healing, but affected stromal cells proliferation, blood and lymphatic vessels formation, and fibrogenesis. Our findings reveal that iron/ferroportin plays a largely underestimated role in the macrophage trophic function in skin homeostasis and repair.
Settore MED/04 - Patologia Generale
16-ago-2018
Article (author)
File in questo prodotto:
File Dimensione Formato  
2018-08 Recalcati Gammella Haematologica.pdf

accesso aperto

Tipologia: Post-print, accepted manuscript ecc. (versione accettata dall'editore)
Dimensione 4.48 MB
Formato Adobe PDF
4.48 MB Adobe PDF Visualizza/Apri
47.full.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 5.43 MB
Formato Adobe PDF
5.43 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/588565
Citazioni
  • ???jsp.display-item.citation.pmc??? 14
  • Scopus 25
  • ???jsp.display-item.citation.isi??? 22
social impact