Resistance of Candida krusei isolates to echinocandins, the recommended drugs to treat candidemia, has been associated with mutations in hot spot (HS) regions of the FKS1 gene or L701 M mutation in a region between HS1 and HS3 of FKS1. However, the role of L701 M mutation alone in causing reduced echinocandins susceptibility is still unclear. We analyzed a region between HS1 and HS3 of FKS1 of 25 C. krusei isolates from clinical samples. Susceptibility to echinocandins was determined by a commercial broth microdilution assay and by the microdilution method according to the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The L701 M mutation was detected in 22/25 (88%) C. krusei isolates with low MIC values in the absence of other HS mutations. The presence of isolated L701 M mutation in C. krusei clinical isolates susceptible to echinocandins suggests that this mutation may be just associated to polymorphism in the C. krusei population.
Presence of L701 M mutation in the FKS1 gene of echinocandin-susceptible Candida krusei isolates / F. Lallitto, A. Prigitano, F. Mangione, A. Piralla, F. Tamarozzi, P. Marone, C. Cavanna. - In: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE. - ISSN 0732-8893. - 92:4(2018 Dec), pp. 311-314.
|Titolo:||Presence of L701 M mutation in the FKS1 gene of echinocandin-susceptible Candida krusei isolates|
|Parole Chiave:||Candida krusei; Candidemia; Echinocandins; FKS1 gene; Microbiology (medical); Infectious Diseases|
|Settore Scientifico Disciplinare:||Settore MED/42 - Igiene Generale e Applicata|
|Data di pubblicazione:||dic-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.diagmicrobio.2018.07.005|
|Appare nelle tipologie:||01 - Articolo su periodico|