The miniature channel, Kcv, is a structural equivalent of the pore of all K+ channels. Here, we follow up on a previous observation that a largely voltage-insensitive channel can be converted into a slow activating inward rectifier after extending the outer transmembrane domain by one Ala. This gain of rectification can be rationalized by dynamic salt bridges at the cytosolic entrance to the channel; opening is favored by voltage-sensitive formation of salt bridges and counteracted by their disruption. Such latent voltage sensitivity in the pore could be relevant for the understanding of voltage gating in complex Kv channels.

Conversion of an instantaneous activating K+ channel into a slow activating inward rectifier / D. Baumeister, B. Hertel, I. Schroeder, S. Gazzarrini, S.M. Kast, J.L. Van Etten, A. Moroni, G. Thiel. - In: FEBS LETTERS. - ISSN 0014-5793. - 591:2(2017), pp. 295-303. [10.1002/1873-3468.12536]

Conversion of an instantaneous activating K+ channel into a slow activating inward rectifier

Schroeder, Indra;Gazzarrini, Sabrina;Moroni, Anna;
2017

Abstract

The miniature channel, Kcv, is a structural equivalent of the pore of all K+ channels. Here, we follow up on a previous observation that a largely voltage-insensitive channel can be converted into a slow activating inward rectifier after extending the outer transmembrane domain by one Ala. This gain of rectification can be rationalized by dynamic salt bridges at the cytosolic entrance to the channel; opening is favored by voltage-sensitive formation of salt bridges and counteracted by their disruption. Such latent voltage sensitivity in the pore could be relevant for the understanding of voltage gating in complex Kv channels.
inward rectification; Kcv K plus channel; salt bridges; voltage-dependent gating
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/585476
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