GABA-B receptors are important for Schwann cell (SC) commitment to a non-myelinating phenotype during development. However, the P0-GABA-B1fl/fl conditional knockout mice, lacking the GABA-B1 receptor specifically in SCs, also presented axon modifications, suggesting SC non-autonomous effects through the neuronal compartment. In this in vitro study, we evaluated whether the specific deletion of the GABA-B1 receptor in SCs may induce autonomous or non-autonomous cross-changes in sensory dorsal root ganglia (DRG) neurons. To this end, we performed an in vitro biomolecular and transcriptomic analysis of SC and DRG neuron primary cultures from P0-GABA-B1fl/fl mice. We found that cells from conditional P0-GABA-B1fl/fl mice exhibited proliferative, migratory and myelinating alterations. Moreover, we found transcriptomic changes in novel molecules that are involved in peripheral neuron-SC interaction.
GABA-B1 Receptor-Null Schwann Cells Exhibit Compromised In Vitro Myelination / A. Faroni, S. Melfi, L.F. Castelnovo, V. Bonalume, D. Colleoni, P. Magni, M.J. Araúzo-Bravo, R. Reinbold, V. Magnaghi. - In: MOLECULAR NEUROBIOLOGY. - ISSN 0893-7648. - 56:2(2019 Feb), pp. 1461-1474.
Titolo: | GABA-B1 Receptor-Null Schwann Cells Exhibit Compromised In Vitro Myelination |
Autori: | MAGNAGHI, VALERIO (Corresponding) |
Parole Chiave: | Conditional mice; GABA-A receptor; Gamma-aminobutyric acid; Peripheral nerve regeneration; |
Settore Scientifico Disciplinare: | Settore BIO/09 - Fisiologia |
Data di pubblicazione: | feb-2019 |
Rivista: | |
Tipologia: | Article (author) |
Data ahead of print / Data di stampa: | 12-giu-2018 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1007/s12035-018-1158-x |
Appare nelle tipologie: | 01 - Articolo su periodico |
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