GABA-B receptors are important for Schwann cell (SC) commitment to a non-myelinating phenotype during development. However, the P0-GABA-B1fl/fl conditional knockout mice, lacking the GABA-B1 receptor specifically in SCs, also presented axon modifications, suggesting SC non-autonomous effects through the neuronal compartment. In this in vitro study, we evaluated whether the specific deletion of the GABA-B1 receptor in SCs may induce autonomous or non-autonomous cross-changes in sensory dorsal root ganglia (DRG) neurons. To this end, we performed an in vitro biomolecular and transcriptomic analysis of SC and DRG neuron primary cultures from P0-GABA-B1fl/fl mice. We found that cells from conditional P0-GABA-B1fl/fl mice exhibited proliferative, migratory and myelinating alterations. Moreover, we found transcriptomic changes in novel molecules that are involved in peripheral neuron-SC interaction.

GABA-B1 Receptor-Null Schwann Cells Exhibit Compromised In Vitro Myelination / A. Faroni, S. Melfi, L.F. Castelnovo, V. Bonalume, D. Colleoni, P. Magni, M.J. Araúzo-Bravo, R. Reinbold, V. Magnaghi. - In: MOLECULAR NEUROBIOLOGY. - ISSN 0893-7648. - 56:2(2019 Feb), pp. 1461-1474. [10.1007/s12035-018-1158-x]

GABA-B1 Receptor-Null Schwann Cells Exhibit Compromised In Vitro Myelination

S. Melfi;L. F. Castelnovo;V. Bonalume;P. Magni;V. Magnaghi
2019-02

Abstract

GABA-B receptors are important for Schwann cell (SC) commitment to a non-myelinating phenotype during development. However, the P0-GABA-B1fl/fl conditional knockout mice, lacking the GABA-B1 receptor specifically in SCs, also presented axon modifications, suggesting SC non-autonomous effects through the neuronal compartment. In this in vitro study, we evaluated whether the specific deletion of the GABA-B1 receptor in SCs may induce autonomous or non-autonomous cross-changes in sensory dorsal root ganglia (DRG) neurons. To this end, we performed an in vitro biomolecular and transcriptomic analysis of SC and DRG neuron primary cultures from P0-GABA-B1fl/fl mice. We found that cells from conditional P0-GABA-B1fl/fl mice exhibited proliferative, migratory and myelinating alterations. Moreover, we found transcriptomic changes in novel molecules that are involved in peripheral neuron-SC interaction.
Conditional mice; GABA-A receptor; Gamma-aminobutyric acid; Peripheral nerve regeneration;
Settore BIO/09 - Fisiologia
12-giu-2018
MOLECULAR NEUROBIOLOGY
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/585342
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