New peptides derived from the natural antimicrobial temporin B were obtained. The design, the antimicrobial activity as well as the characterization of the secondary structure of peptides in the presence of bacterial cells is here described. We identified TB_KKG6K (KKLLPIVKNLLKSLL) as the most active analogue against Gram-positive and Gram-negative bacteria as compared to natural temporin B (LLPIVGNLLKSLL) and TB_KKG6A (KKLLPIVANLLKSLL). Acylation with hydrophobic moieties led generally to reduced activity, however, acylation at position 6 of TB_KKG6K led to retained submicromolar activity against Staphylococcus epidermidis.
Effect of acylation on the antimicrobial activity of temporin B analogues / C. Avitabile, L.D. D'Andrea, E. D'Aversa, R. Milani, R. Gambari, A. Romanelli. - In: CHEMMEDCHEM. - ISSN 1860-7179. - 13:15(2018 Aug 10), pp. 1549-1554.
Titolo: | Effect of acylation on the antimicrobial activity of temporin B analogues |
Autori: | ROMANELLI, ALESSANDRA (Ultimo) (Corresponding) |
Parole Chiave: | acylation; antimicrobial activity; peptides; secondary structures; substituent effects; antimicrobial; acylatio;; circular dichroism; E. coli ; S. epidermidis |
Settore Scientifico Disciplinare: | Settore CHIM/03 - Chimica Generale e Inorganica |
Data di pubblicazione: | 10-ago-2018 |
Rivista: | |
Tipologia: | Article (author) |
Data ahead of print / Data di stampa: | 19-giu-2018 |
Digital Object Identifier (DOI): | http://dx.doi.org/10.1002/cmdc.201800289 |
Appare nelle tipologie: | 01 - Articolo su periodico |
File in questo prodotto:
File | Descrizione | Tipologia | Licenza | |
---|---|---|---|---|
Manuscript 28042018.pdf | Pre-print (manoscritto inviato all'editore) | Open Access Visualizza/Apri | ||
Avitabile_EtAlii.pdf | Post-print, accepted manuscript ecc. (versione accettata dall'editore) | Open Access Visualizza/Apri | ||
cmdc.201800289.pdf | Publisher's version/PDF | Administrator Richiedi una copia |