Staphylococcus aureus is recognized worldwide as one of the major agents of dairy cow intra-mammary infections. This microorganism can express a wide spectrum of pathogenic factors used to attach, colonize, invade and infect the host. The present study evaluated 120 isolates from eight different countries that were genotyped by RS-PCR and investigated for 26 different virulence factors to increase the knowledge on the circulating genetic lineages among the cow population with mastitis. New genotypes were observed for South African strains while for all the other countries new variants of existing genotypes were detected. For each country, a specific genotypic pattern was found. Among the virulence factors, fmtB, cna, clfA and leucocidins genes were the most frequent. The sea and sei genes were present in seven out of eight countries; seh showed high frequency in South American countries (Brazil, Colombia, Argentina), while sel was harboured especially in one Mediterranean country (Tunisia). The etb, seb and see genes were not detected in any of the isolates, while only two isolates were MRSA (Germany and Italy) confirming the low diffusion of methicillin resistance microorganism among bovine mastitis isolates. This work demonstrated the wide variety of S. aureus genotypes found in dairy cattle worldwide. This condition suggests that considering the region of interest might help to formulate strategies for reducing the infection spreading.

Staphylococcus aureus Isolates from Bovine Mastitis in Eight Countries: Genotypes, Detection of Genes Encoding Different Toxins and Other Virulence Genes / V. Monistero, H.U. Graber, C. Pollera, P. Cremonesi, B. Castiglioni, E. Bottini, A. Ceballos-Marquez, L. Lasso-Rojas, V. Kroemker, N. Wente, I. Petzer, C. Santisteban, J. Runyan, M. Veiga Dos Santos, B.G. Alves, R. Piccinini, V. Bronzo, M.S. Abbassi, M.B. Said, P. Moroni. - In: TOXINS. - ISSN 2072-6651. - 10:6(2018 Jun 17), pp. 247.1-247.22. [10.3390/toxins10060247]

Staphylococcus aureus Isolates from Bovine Mastitis in Eight Countries: Genotypes, Detection of Genes Encoding Different Toxins and Other Virulence Genes

V. Monistero;C. Pollera;R. Piccinini;V. Bronzo;P. Moroni
Ultimo
2018

Abstract

Staphylococcus aureus is recognized worldwide as one of the major agents of dairy cow intra-mammary infections. This microorganism can express a wide spectrum of pathogenic factors used to attach, colonize, invade and infect the host. The present study evaluated 120 isolates from eight different countries that were genotyped by RS-PCR and investigated for 26 different virulence factors to increase the knowledge on the circulating genetic lineages among the cow population with mastitis. New genotypes were observed for South African strains while for all the other countries new variants of existing genotypes were detected. For each country, a specific genotypic pattern was found. Among the virulence factors, fmtB, cna, clfA and leucocidins genes were the most frequent. The sea and sei genes were present in seven out of eight countries; seh showed high frequency in South American countries (Brazil, Colombia, Argentina), while sel was harboured especially in one Mediterranean country (Tunisia). The etb, seb and see genes were not detected in any of the isolates, while only two isolates were MRSA (Germany and Italy) confirming the low diffusion of methicillin resistance microorganism among bovine mastitis isolates. This work demonstrated the wide variety of S. aureus genotypes found in dairy cattle worldwide. This condition suggests that considering the region of interest might help to formulate strategies for reducing the infection spreading.
S. aureus; dairy cow; genotypes; mastitis; virulence genes
Settore VET/05 - Malattie Infettive degli Animali Domestici
Article (author)
File in questo prodotto:
File Dimensione Formato  
4. Staphylococcus aures isolated from bovine mastits in Eight Countries Genotypes, Detection of Genes.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 537.57 kB
Formato Adobe PDF
537.57 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/579562
Citazioni
  • ???jsp.display-item.citation.pmc??? 29
  • Scopus 53
  • ???jsp.display-item.citation.isi??? 53
social impact