Proteomics methodologies hold great promise in basic renal research and clinical nephrology. The classical approach for proteomic analysis couples two-dimensional gel electrophoresis (2-DE) with protein identification by mass spectrometry to produce more global information regarding normal protein expression and alterations in different physiological and pathological states. In this report we have expanded the identification of proteins in the renal cortex, improving the previously published map to facilitate the study of different diseases affecting the human kidney. About 250 spots were analyzed by peptide mass fingerprinting, 89 proteins and 74 isoforms for some of them were identified and implemented in the normal human renal cortex 2DE reference map. This more comprehensive view of the proteome of the human renal cortex could be of invaluable help to the differential proteomic display of urological diseases.
|Titolo:||Expanding the proteome two-dimensional gel electrophoresis reference map of human renal cortex by peptide mass fingerprinting|
|Parole Chiave:||human renal cortex; kidney master image; mass spectrometry; two-dimensional gel electrophoresis; peptide mass fingerprinting|
|Settore Scientifico Disciplinare:||Settore MED/08 - Anatomia Patologica|
Settore MED/24 - Urologia
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||10.1002/pmic.200401077|
|Appare nelle tipologie:||01 - Articolo su periodico|