Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue specimens is a widely used laboratory technique that allows the demonstration and the direct microscopical observation of antigens within tissue sections by means of specific antibodies. IHC is one the best tools to define the immunophenotype of a cell during maturation or neoplastic transformation.To investigate the immunophenotype of postnatal rabbit Sertoli cell, from neonatal to adult age, in this chapter, the immunohistochemical protocol described is one of the most common: the avidin-biotin complex (ABC) method.
Rabbit Sertoli cells: Immunohistochemical profile from neonatal to adult age / V. Grieco, B. Banco (METHODS IN MOLECULAR BIOLOGY). - In: Sertoli cell / [a cura di] M.G. Alves, P.F. Oliveira. - [s.l] : Humana Press, 2018. - ISBN 9781493976973. - pp. 37-47 [10.1007/978-1-4939-7698-0_4]
Rabbit Sertoli cells: Immunohistochemical profile from neonatal to adult age
V. GriecoPrimo
;B. BancoUltimo
2018
Abstract
Immunohistochemistry (IHC) on formalin-fixed paraffin-embedded tissue specimens is a widely used laboratory technique that allows the demonstration and the direct microscopical observation of antigens within tissue sections by means of specific antibodies. IHC is one the best tools to define the immunophenotype of a cell during maturation or neoplastic transformation.To investigate the immunophenotype of postnatal rabbit Sertoli cell, from neonatal to adult age, in this chapter, the immunohistochemical protocol described is one of the most common: the avidin-biotin complex (ABC) method.
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