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EnglishAfter hind limb suspension, a remodeling of postural muscle phenotype is observed. This remodeling results in a shift of muscle profile from slow-oxidative to fast-glycolytic. These metabolic changes and fiber type shift increase muscle fatigability. Acetyl-L-carnitine (ALCAR) influences the skeletal muscle phenotype of soleus muscle suggesting a positive role of dietary supplementation of ALCAR during unloading. In the present study, we applied a 2-D DIGE, mass spectrometry and biochemical assays, to assess qualitative and quantitative differences in the proteome of rat slow-twitch soleus muscle subjected to disuse. Meanwhile, the effects of ALCAR administration on muscle proteomic profile in both unloading and normal-loading conditions were evaluated. The results indicate a modulation of troponin I and tropomyosin complex to regulate fiber type transition. Associated, or induced, metabolic changes with an increment of glycolytic enzymes and a decreased capacity of fat oxidation are observed. These metabolic changes appear to be counteracted by ALCAR treatment, which restores the mitochondrial mass and decreases the glycolytic enzyme expression, suggesting a normalization of the metabolic shift observed in unloaded animals. This normalization is accompanied by a maintenance of body weight and seems to prevent a switch of fiber type.
| Titolo: | A DIGE approach for the assessment of rat soleus muscle changes during unloading : effect of acetyl-L-carnitine supplementation |
| Autori: | MORIGGI, MANUELA (Primo) GELFI, CECILIA (Ultimo) |
| Parole Chiave: | Carbonic-Anhydrase-III ; Difference Gel-Electrophoresis ; Skeletal-Muscle ; Simulated Microgravity ; Hindlimb Suspension ; Protein Expression ; Proteomic Analysis ; Gene-Expression ; Disuse Atrophy ; Old Rats ; Acetyl-L-carnitine ; Mass spectrometry ; Two-dimensional in-gel electrophoresis ; Unloading |
| Settore Scientifico Disciplinare: | Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica |
| Data di pubblicazione: | set-2008 |
| Rivista: | |
| Tipologia: | Article (author) |
| Digital Object Identifier (DOI): | 10.1002/pmic.200701176 |
| Appare nelle tipologie: | 01 - Articolo su periodico |
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