CONTEXT: - Molecular testing is essential for the diagnostic workup of patients with advanced non-small cell lung cancers. Cytology specimens from minimally invasive procedures, such as endobronchial ultrasound-guided transbronchial needle aspiration, are often the only available samples for these patients. The implementation of molecular diagnostic testing, and in particular next-generation sequencing-based testing, on these cytologic specimens is currently an evolving field for lung cytopathology. The application of these molecular analyses on tyrosine kinase inhibitor-resistant non-small cell lung cancers raises unique technical, biologic, and clinical challenges. OBJECTIVE: - To provide an overview of the implementation of next-generation sequencing analysis on endobronchial ultrasound-guided transbronchial needle aspiration samples to detect the molecular aberrations underneath the phenomenon of acquired resistance in patients with non-small cell lung cancers progressing while on the EGFR/ALK tyrosine kinase inhibitor treatment. DATA SOURCES: - Peer-reviewed original articles, review articles, and published guidelines and expert opinion reports were reviewed, together with our single-center experience. CONCLUSIONS: - Next-generation sequencing analyses and the endobronchial ultrasound-guided transbronchial needle aspiration procedure may represent a valuable strategy to address the unique requirements of molecular testing on tyrosine kinase inhibitor-resistant non-small cell lung cancers.
Acquired Resistance to Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancers : the Role of Next-Generation Sequencing on Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Samples / E. Guerini-Rocco, A. Passaro, C. Casadio, V.M. De Luca, J. Guarize, F. de Marinis, D. Vacirca, M. Barberis. - In: ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE. - ISSN 0003-9985. - 142:4(2018 Apr), pp. 465-473.
|Titolo:||Acquired Resistance to Tyrosine Kinase Inhibitors in Non-Small Cell Lung Cancers : the Role of Next-Generation Sequencing on Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration Samples|
GUERINI ROCCO, ELENA (Corresponding)
|Settore Scientifico Disciplinare:||Settore MED/08 - Anatomia Patologica|
|Data di pubblicazione:||apr-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.5858/arpa.2017-0158-RA|
|Appare nelle tipologie:||01 - Articolo su periodico|