Arabidopsis thaliana seeds comprise three tissues, which are genetically and functionally distinct. However, in order to ensure a correct seed development, a communication and coordination of them is strictly necessary. The seed coat is a five-layer tissue surrounding the female gametophyte. In the stk abs double mutant only four layers form this tissue: the endothelium, the innermost one, does not differentiate. Since the endothelium is a sporophytic layer directly in contact with the gametophyte before the fertilization and with the endosperm after the fertilization, we hypothesized a possible role of this layer in the communication between those tissues. In this work, the role of the endothelium has been investigated, as well as the role of the STK and ABS, the two MADS box transcription factors required for endothelium differentiation. An additional interesting phenotype of the stk abs double mutant is the excessive amount of starch accumulating in the central cell of the female gametophyte. This might causes the partial sterility described for the double mutant. Combining stk abs with the gpt1 mutant, whose mature ovules do not accumulate starch, we found a partial recovery of the phenotype; this result support the idea that the excess of starch in the double mutant could physically prevent the correct fertilization process.

GAMETOPHYTE AND SPOROPHYTE CROSSTALK DURING FERTILIZATION IN ARABIDOPSIS / G. Leo ; scientific tutor: L. Colombo. - : . DIPARTIMENTO DI BIOSCIENZE, 2018 Feb 23. ((30. ciclo, Anno Accademico 2017. [10.13130/leo-giulia_phd2018-02-23].

GAMETOPHYTE AND SPOROPHYTE CROSSTALK DURING FERTILIZATION IN ARABIDOPSIS

LEO, GIULIA
2018-02-23

Abstract

Arabidopsis thaliana seeds comprise three tissues, which are genetically and functionally distinct. However, in order to ensure a correct seed development, a communication and coordination of them is strictly necessary. The seed coat is a five-layer tissue surrounding the female gametophyte. In the stk abs double mutant only four layers form this tissue: the endothelium, the innermost one, does not differentiate. Since the endothelium is a sporophytic layer directly in contact with the gametophyte before the fertilization and with the endosperm after the fertilization, we hypothesized a possible role of this layer in the communication between those tissues. In this work, the role of the endothelium has been investigated, as well as the role of the STK and ABS, the two MADS box transcription factors required for endothelium differentiation. An additional interesting phenotype of the stk abs double mutant is the excessive amount of starch accumulating in the central cell of the female gametophyte. This might causes the partial sterility described for the double mutant. Combining stk abs with the gpt1 mutant, whose mature ovules do not accumulate starch, we found a partial recovery of the phenotype; this result support the idea that the excess of starch in the double mutant could physically prevent the correct fertilization process.
COLOMBO, LUCIA
Settore BIO/11 - Biologia Molecolare
GAMETOPHYTE AND SPOROPHYTE CROSSTALK DURING FERTILIZATION IN ARABIDOPSIS / G. Leo ; scientific tutor: L. Colombo. - : . DIPARTIMENTO DI BIOSCIENZE, 2018 Feb 23. ((30. ciclo, Anno Accademico 2017. [10.13130/leo-giulia_phd2018-02-23].
Doctoral Thesis
File in questo prodotto:
File Dimensione Formato  
phd_unimi_R10952.pdf

embargo fino al 25/05/2020

Descrizione: tesi completa
Tipologia: Tesi di dottorato completa
Dimensione 2.66 MB
Formato Adobe PDF
2.66 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/565198
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact