Salinity tolerance has been extensively investigated in recent years due to its agricultural importance. Several features, such as the regulation of ionic transporters and metabolic adjustments, have been identified as salt tolerance hallmarks. Nevertheless, due to the complexity of the trait, the results achieved to date have met with limited success in improving the salt tolerance of rice plants when tested in the field, thus suggesting that a better understanding of the tolerance mechanisms is still required. In this work, differences between two varieties of rice with contrasting salt sensitivities were revealed by the imaging of photosynthetic parameters, ion content analysis and a transcriptomic approach. The transcriptomic analysis conducted on tolerant plants supported the setting up of an adaptive program consisting of sodium distribution preferentially limited to the roots and older leaves, and in the activation of regulatory mechanisms of photosynthesis in the new leaves. As a result, plants resumed grow even under prolonged saline stress. In contrast, in the sensitive variety, RNA-seq analysis revealed a misleading response, ending in senescence and cell death. The physiological response at the cellular level was investigated by measuring the intracellular profile of H2O2in the roots, using a fluorescent probe. In the roots of tolerant plants, a quick response was observed with an increase in H2O2production within 5 min after salt treatment. The expression analysis of some of the genes involved in perception, signal transduction and salt stress response confirmed their early induction in the roots of tolerant plants compared to sensitive ones. By inhibiting the synthesis of apoplastic H2O2, a reduction in the expression of these genes was detected. Our results indicate that quick H2O2signaling in the roots is part of a coordinated response that leads to adaptation instead of senescence in salt-treated rice plants.

Transcriptome and cell physiological analyses in different rice cultivars provide new insights into adaptive and salinity stress responses / E. Formentin, C. Sudiro, G. Perin, S. Riccadonna, E. Barizza, E. Baldoni, E. Lavezzo, P. Stevanato, G.A. Sacchi, P. Fontana, S. Toppo, T. Morosinotto, M. Zottini, F. Lo Schiavo. - In: FRONTIERS IN PLANT SCIENCE. - ISSN 1664-462X. - 9(2018 Mar 05).

Transcriptome and cell physiological analyses in different rice cultivars provide new insights into adaptive and salinity stress responses

E. Baldoni;G.A. Sacchi;P. Fontana;
2018

Abstract

Salinity tolerance has been extensively investigated in recent years due to its agricultural importance. Several features, such as the regulation of ionic transporters and metabolic adjustments, have been identified as salt tolerance hallmarks. Nevertheless, due to the complexity of the trait, the results achieved to date have met with limited success in improving the salt tolerance of rice plants when tested in the field, thus suggesting that a better understanding of the tolerance mechanisms is still required. In this work, differences between two varieties of rice with contrasting salt sensitivities were revealed by the imaging of photosynthetic parameters, ion content analysis and a transcriptomic approach. The transcriptomic analysis conducted on tolerant plants supported the setting up of an adaptive program consisting of sodium distribution preferentially limited to the roots and older leaves, and in the activation of regulatory mechanisms of photosynthesis in the new leaves. As a result, plants resumed grow even under prolonged saline stress. In contrast, in the sensitive variety, RNA-seq analysis revealed a misleading response, ending in senescence and cell death. The physiological response at the cellular level was investigated by measuring the intracellular profile of H2O2in the roots, using a fluorescent probe. In the roots of tolerant plants, a quick response was observed with an increase in H2O2production within 5 min after salt treatment. The expression analysis of some of the genes involved in perception, signal transduction and salt stress response confirmed their early induction in the roots of tolerant plants compared to sensitive ones. By inhibiting the synthesis of apoplastic H2O2, a reduction in the expression of these genes was detected. Our results indicate that quick H2O2signaling in the roots is part of a coordinated response that leads to adaptation instead of senescence in salt-treated rice plants.
H2O2; ion transporters; Oryza sativa (rice); RNA sequencing; salt stress; salt tolerance mechanisms; plant science
Settore AGR/13 - Chimica Agraria
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/564479
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