The cestode Molicola horridus is a muscle parasite of teleost ﬁsh. The ability of molecules present in this parasite to induce allergic response is not known yet. Since ﬁsh-borne parasitic allergens can induce allergic manifestations even when the parasitized ﬁsh is well cooked, the knowledge of potential allergens present in food is important in order to provide a save products for consumers. The aim of the study was to determine the allergenic potential of the components present in the crude larval extract (CLE) of M. horridus. Two mouse models were exposed to the CLE: adult BALB/c mice that were intraperitoneally (i.p.)immunized and newborn BALB/c mice that were orally exposed. Speciﬁc antibody levels in serum and faeces were measured by ELISA. The cellular immune response was determined by proliferation assay of splenocytes from sensitized mice. The protein proﬁle of CLE was analysed by SDS-PAGE and western blot. In adult mice, speciﬁc IgG and IgA were detected in sera and faeces, whereas speciﬁc IgE were detected in sera only. In newborn mice, speciﬁc IgG were detected in sera and a low level of IgA was detected in faeces. SDS-PAGE revealed the CLE protein proﬁle, with most of the roteins running from 15 to 50 kDa. Speciﬁc IgG recognized mainly the 26 and 75 kDa proteins and a molecular complex below 100 kDa by immunoblot. Speciﬁc IgE recognized the same 26 kDa protein as IgG did, and, with less intensity, another protein at 30 kDa. Splenocytes from CLE-immunized mice proliferated when stimulated with CLE in a dose-dependent manner. The crude larval extract from M. horridus has potential allergenic molecules which can represent a risk for ﬁsh consumers.
|Titolo:||Allergenic activity of Molicola horridus (Cestoda, Trypanorhyncha), a cosmopolitan fish parasite, in a mouse model|
PICCOLO, GIULIANA (Penultimo)
|Parole Chiave:||balb/c mice; fish allergens; food allergy; molicola horridus; mouse model; swordfish|
|Settore Scientifico Disciplinare:||Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali|
|Data di pubblicazione:||7-nov-2008|
|Digital Object Identifier (DOI):||10.1016/j.vetpar.2008.07.010|
|Appare nelle tipologie:||01 - Articolo su periodico|