The B203.13 monoclonal antibody was developed by immunizing mice with the B/monocyte biphenotypic cell line B1b. During normal hematopoiesis B203.13 is expressed on a fraction of CD34+ cells, while on mature cells it is only present on B-lymphocytes. We tested this antibody as a marker of childhood B-acute lymphoblastic leukemia (B-ALL). Bone marrow aspirates from 139 cases of early B-ALL and 25 controls were studied. About 40% of the B-ALL patients expressed B203.13. In these patients, B203.13 was constantly co-expressed with CD10, but never co-expressed with CD20, contrary to the controls. The CD10(+)/B203.13(+) phenotype was specific to B-ALL, since CD10(+)/CD20(+) cells from common acute lymphoblastic leukemia (c-ALL) did not express B203.13. We concluded that the use of B203.13 in association with CD10 and CD20 provides meaningful information for distinguishing normal residual B-cells from leukemic B-lymphoblasts and that recurrence of a CD10(+)/B203.13(+) phenotype after transplantation may be a very early relapse indicator of early B-acute lymphoblastic leukemia.
Aberrant expression of B203.13 antigen in acute lymphoid leukemia of B-cell origin / G. Gobbi, P. Mirandola, C. Malinverno, I. Sponzilli, C. Carubbi, F. Ricci, R. Binazzi, G. Basso, G. Giuliani Piccari, G. Ramazzotti, G. Pasquantonio, L. Cocco, M. Vitale. - In: INTERNATIONAL JOURNAL OF ONCOLOGY. - ISSN 1019-6439. - 33:2(2008 Aug), pp. 371-374.
|Titolo:||Aberrant expression of B203.13 antigen in acute lymphoid leukemia of B-cell origin|
|Parole Chiave:||adolescent; adult; antigens, cd20; antigens, neoplasm; child; child, preschool; female; flow cytometry; humans; immunophenotyping; infant; male; neprilysin; precursor B-cell lymphoblastic leukemia-lymphoma|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||ago-2008|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3892/ijo_00000017|
|Appare nelle tipologie:||01 - Articolo su periodico|