The toxic effects of exogenous hydrogen sulfide on peripheral blood lymphocytes have been investigated in detail. Hydrogen sulfide is now considered as a gasotransmitter with specific functional roles in different cell types, like neurons and vascular smooth muscle. Here we show that exogenous hydrogen sulfide induces a caspase-independent cell death of peripheral blood lymphocytes that depends on their intracellular glutathion levels, with a physiologically relevant subset specificity for CD8+ T cells and NK cells. Although lymphocyte activation does not modify their sensitivity to HS-, after 24 h exposure to hydrogen sulfide surviving lymphocyte subsets show a dramatically decreased proliferation in response to mitogens and a reduced IL-2 production. Overall, our data demonstrate that HS- reduces the cellular cytotoxic response of peripheral blood lymphocytes as well as their production of IL-2, therefore de-activating the major players of local inflammatory responses, adding new basic knowledge to the clinically well known anti-inflammatory effects of sulfur compounds.
Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets / P. Mirandola, G. Gobbi, I. Sponzilli, M. Pambianco, C. Malinverno, A. Cacchioli, G. De Panfilis, M. Vitale. - In: JOURNAL OF CELLULAR PHYSIOLOGY. - ISSN 0021-9541. - 213:3(2007 Dec), pp. 826-833.
|Titolo:||Exogenous hydrogen sulfide induces functional inhibition and cell death of cytotoxic lymphocytes subsets|
|Parole Chiave:||T-cells; psoriasis-vulgaris; flow-cytometry; endogenous production; apoptosis; H2S; glutathione; modulation; microscopy; mammals|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||dic-2007|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1002/jcp.21151|
|Appare nelle tipologie:||01 - Articolo su periodico|
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