The aim of this study was to evaluate the potential toxicological effects on fish related to the leakage of yperite from rusted bomb shells dumped at sea. Both in vivo and field studies have been performed. As for the in vivo experiment, specimen of European eel were subcutaneously injected with 0.015, 0.15 and 1.5. mg/kg of yperite and sacrificed after 24 and 48. h. In the field study, specimen of Conger eel were collected from a dumping site in the Southern Adriatic Sea. The presence/absence of yperite in tissues, genotoxicity, detoxification enzymes, histological alterations and gross abnormalities were investigated. Results of the in vivo experiment showed a significant increase of EROD activity at both 24. h and 48. h. UGT activity increased significantly at 48. h post injection. An acute inflammatory response after 24. h in skin layers and muscle was observed, associated to cell degeneration and necrosis after 48. h at the highest dose. On field, comet assay revealed genotoxicity in gills of fish from the dumping site. Specimen from the dumping site showed significantly higher EROD activities compared to controls, deep ulcers and papules on skin together with liver and spleen histopathological lesions.
|Titolo:||Environmental hazard of yperite released at sea : sublethal toxic effects on fish|
|Parole Chiave:||Genotoxicity; Histopathology; Integrated ecotoxicological approach; Yperite; Animals; Cytochrome P-450 CYP1A1; Fish Proteins; Glucuronosyltransferase; Glutathione Transferase; Micronucleus Tests; Muscle, Skeletal; Mustard Gas; Oceans and Seas; Skin; Water Pollutants, Chemical; Anguilla; Environmental Engineering; Environmental Chemistry; Waste Management and Disposal; Pollution; Health, Toxicology and Mutagenesis|
|Settore Scientifico Disciplinare:||Settore BIO/07 - Ecologia|
|Data di pubblicazione:||2013|
|Digital Object Identifier (DOI):||10.1016/j.jhazmat.2013.01.003|
|Appare nelle tipologie:||01 - Articolo su periodico|