Due to the large production and growing use of titanium dioxide nanoparticles (n-TiO2), their release in the marine environment and their potential interaction with existing toxic contaminants represent a growing concern for biota. Different end-points of genotoxicity were investigated in the European sea bass Dicentrarchus labrax exposed to n-TiO2(1mgL-1) either alone and combined with CdCl2(0.1mgL-1) for 7 days. DNA primary damage (comet assay), apoptotic cells (diffusion assay), occurrence of micronuclei and nuclear abnormalities (cytome assay) were assessed in peripheral erythrocytes and genomic stability (random amplified polymorphism DNA-PCR, RAPD assay) in muscle tissue. Results showed that genome template stability was reduced after CdCl2and n-TiO2exposure. Exposure to n-TiO2alone was responsible for chromosomal alteration but ineffective in terms of DNA damage while the opposite was observed in CdCl2exposed specimens. Co-exposure apparently prevents the chromosomal damage and leads to a partial recovery of the genome template stability.
n-TiO2and CdCl2co-exposure to titanium dioxide nanoparticles and cadmium : genomic, DNA and chromosomal damage evaluation in the marine fish European sea bass (Dicentrarchus labrax) / M. Nigro, M. Bernardeschi, D. Costagliola, C. Della Torre, G. Frenzilli, P. Guidi, P. Lucchesi, F. Mottola, M. Santonastaso, V. Scarcelli, F. Monaci, I. Corsi, V. Stingo, L. Rocco. - In: AQUATIC TOXICOLOGY. - ISSN 0166-445X. - 168(2015), pp. 72-77. [10.1016/j.aquatox.2015.09.013]
n-TiO2and CdCl2co-exposure to titanium dioxide nanoparticles and cadmium : genomic, DNA and chromosomal damage evaluation in the marine fish European sea bass (Dicentrarchus labrax)
C. Della Torre;
2015
Abstract
Due to the large production and growing use of titanium dioxide nanoparticles (n-TiO2), their release in the marine environment and their potential interaction with existing toxic contaminants represent a growing concern for biota. Different end-points of genotoxicity were investigated in the European sea bass Dicentrarchus labrax exposed to n-TiO2(1mgL-1) either alone and combined with CdCl2(0.1mgL-1) for 7 days. DNA primary damage (comet assay), apoptotic cells (diffusion assay), occurrence of micronuclei and nuclear abnormalities (cytome assay) were assessed in peripheral erythrocytes and genomic stability (random amplified polymorphism DNA-PCR, RAPD assay) in muscle tissue. Results showed that genome template stability was reduced after CdCl2and n-TiO2exposure. Exposure to n-TiO2alone was responsible for chromosomal alteration but ineffective in terms of DNA damage while the opposite was observed in CdCl2exposed specimens. Co-exposure apparently prevents the chromosomal damage and leads to a partial recovery of the genome template stability.Pubblicazioni consigliate
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