Due to the large production and growing use of titanium dioxide nanoparticles (n-TiO2), their release in the marine environment and their potential interaction with existing toxic contaminants represent a growing concern for biota. Different end-points of genotoxicity were investigated in the European sea bass Dicentrarchus labrax exposed to n-TiO2(1mgL-1) either alone and combined with CdCl2(0.1mgL-1) for 7 days. DNA primary damage (comet assay), apoptotic cells (diffusion assay), occurrence of micronuclei and nuclear abnormalities (cytome assay) were assessed in peripheral erythrocytes and genomic stability (random amplified polymorphism DNA-PCR, RAPD assay) in muscle tissue. Results showed that genome template stability was reduced after CdCl2and n-TiO2exposure. Exposure to n-TiO2alone was responsible for chromosomal alteration but ineffective in terms of DNA damage while the opposite was observed in CdCl2exposed specimens. Co-exposure apparently prevents the chromosomal damage and leads to a partial recovery of the genome template stability.
n-TiO2and CdCl2co-exposure to titanium dioxide nanoparticles and cadmium : genomic, DNA and chromosomal damage evaluation in the marine fish European sea bass (Dicentrarchus labrax) / M. Nigro, M. Bernardeschi, D. Costagliola, C. Della Torre, G. Frenzilli, P. Guidi, P. Lucchesi, F. Mottola, M. Santonastaso, V. Scarcelli, F. Monaci, I. Corsi, V. Stingo, L. Rocco. - In: AQUATIC TOXICOLOGY. - ISSN 0166-445X. - 168(2015), pp. 72-77.
|Titolo:||n-TiO2and CdCl2co-exposure to titanium dioxide nanoparticles and cadmium : genomic, DNA and chromosomal damage evaluation in the marine fish European sea bass (Dicentrarchus labrax)|
|Parole Chiave:||CdCl2; Dicentrarchus labrax; DNA damage; Genotoxicity; Micronuclei; Nano-TiO2; Animals; Bass; Cadmium; Cadmium Chloride; Chromosomes; Comet Assay; DNA; Genome; Genomics; Nanoparticles; Random Amplified Polymorphic DNA Technique; Titanium; Water Pollutants, Chemical; DNA Damage; Aquatic Science; Health, Toxicology and Mutagenesis|
|Settore Scientifico Disciplinare:||Settore BIO/07 - Ecologia|
|Data di pubblicazione:||2015|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.aquatox.2015.09.013|
|Appare nelle tipologie:||01 - Articolo su periodico|