Calcium sensors are indispensable tools to study the role of Ca2+and visualize Ca2+dynamics during biological processes. Over the past years, the field of Ca2+imaging has strongly expanded by the development of a wide palette of sensors and optimization of sample handling. Here, we provide guidelines for imaging of the Ca2+sensor R-GECO1 in Arabidopsis thaliana roots which can be interpolated to other intensiometric Ca2+sensors. Furthermore, we demonstrate a procedure for image analysis of the acquired time-lapse recordings.

Calcium Ion Dynamics in Roots: Imaging and Analysis / E. Himschoot, M. Krebs, A. Costa, T. Beeckman, S. Vanneste (METHODS IN MOLECULAR BIOLOGY). - In: Root Development / [a cura di] D. Ristova, E. Barbez. - [s.l] : Springer, 2018. - ISBN 978-1-4939-7746-8. - pp. 115-130 [10.1007/978-1-4939-7747-5_9]

Calcium Ion Dynamics in Roots: Imaging and Analysis

A. Costa;
2018

Abstract

Calcium sensors are indispensable tools to study the role of Ca2+and visualize Ca2+dynamics during biological processes. Over the past years, the field of Ca2+imaging has strongly expanded by the development of a wide palette of sensors and optimization of sample handling. Here, we provide guidelines for imaging of the Ca2+sensor R-GECO1 in Arabidopsis thaliana roots which can be interpolated to other intensiometric Ca2+sensors. Furthermore, we demonstrate a procedure for image analysis of the acquired time-lapse recordings.
Arabidopsis thaliana; Ca2+ imaging; Confocal laser scanning microscopy; Fiji; MATLAB; R-GECO1; Spinning disk microscopy
Settore BIO/04 - Fisiologia Vegetale
2018
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/561700
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