A simple and rapid high-performance liquid chromatographic method with ultraviolet detection was developed for the quantitative determination of retigabine, known also as ezogabine, in human plasma. The assay uses a simple solid-phase extraction for sample preparation and direct injection of the extract into the chromatograph. Flupirtine is used as an internal standard. Chromatographic separation is achieved on a C18 Chromolith column (Chromolith Performance, 100 × 4.6 mm i.d.), using as mobile phase water/acetonitrile/methanol (72:18:10 v/v/v) mixed with 0.1% of 85% phosphoric acid. Isocratic elution is conducted at a flow rate of 1.5 mL min-1 . The total duration of a chromatographic run is 7 min. Calibration curves are linear over the 25-2000 ng mL-1 concentration range, with a limit of quantitation of 25 ng mL-1 . Other performance characteristics include high precision (intra- and inter-day coefficients of variation ≤12.6%) and high accuracy (99.7%-108.7%). The method is suitable for the investigation of concentration-response relationships in patients receiving therapeutic doses of retigabine.
|Titolo:||A simple and rapid HPLC-UV method for the determination of retigabine in human plasma|
|Parole Chiave:||Bioanalytical method validation; Ezogabine; HPLC-UV; Retigabine; Solid-phase extraction; Analytical Chemistry; Biochemistry; Molecular Biology; Pharmacology; Drug Discovery; 3003; Pharmaceutical Science; Clinical Biochemistry|
|Settore Scientifico Disciplinare:||Settore MED/39 - Neuropsichiatria Infantile|
|Data di pubblicazione:||mag-2018|
|Digital Object Identifier (DOI):||10.1002/bmc.4168|
|Appare nelle tipologie:||01 - Articolo su periodico|