1. Aim of this study was the development of an optimised cryopreservation pellet procedure for chicken semen and the assessment of DNA and membrane integrity in frozen/thawed spermatozoa in a Hubbard F15 meat type selected strain.2. The following semen processing conditions were studied: spermatozoa working concentration (SWC), 1.5 vs 2x10(9) cells/ml in pre-freezing extender; equilibration of diluted semen at 5 degrees C, 20 vs 40min; dimethylacetamide concentration, 6% vs 9%; dimethylacetamide equilibration time at 5 degrees C, 1 vs 30min; thawing at 60 degrees C for 10 vs 50 degrees C for 30sec. Spermatozoa viability (EtBr exclusion procedure - stress test), mobility (Accudenz (R) swim-down test) and subjective motility were assessed in fresh and frozen-thawed semen.3. The lower SWC (1.5 x 10(9) cells/ml) and the higher dimethylacetamide concentration (9%) had positive significant effects on the recovery rate of motile (22% vs 16%) and viable spermatozoa (39 vs 34%), respectively.4.Membrane (SYBR14-PI staining) and DNA integrity (comet assay) were assessed before and after freezing/thawing according to the optimised protocol.5. Recovery rates of spermatozoa with undamaged plasma membrane and DNA were 41% and 76%, respectively. The distribution of spermatozoa in classes of DNA damage was also analysed and discussed.6. It was concluded that pellet cryopreservation was a damaging process mainly for plasma membrane rather than nuclear DNA in chicken spermatozoa.

Spermatozoa DNA and plasma membrane integrity after pellet optimized processing for cryopreservation in meat type chicken breeders / T.M. Gliozzi, L. Zaniboni, N. Iaffaldano, S. Cerolini. - In: BRITISH POULTRY SCIENCE. - ISSN 0007-1668. - 58:5(2017), pp. 578-584.

Spermatozoa DNA and plasma membrane integrity after pellet optimized processing for cryopreservation in meat type chicken breeders

L. Zaniboni;S. Cerolini
Membro del Collaboration Group
2017

Abstract

1. Aim of this study was the development of an optimised cryopreservation pellet procedure for chicken semen and the assessment of DNA and membrane integrity in frozen/thawed spermatozoa in a Hubbard F15 meat type selected strain.2. The following semen processing conditions were studied: spermatozoa working concentration (SWC), 1.5 vs 2x10(9) cells/ml in pre-freezing extender; equilibration of diluted semen at 5 degrees C, 20 vs 40min; dimethylacetamide concentration, 6% vs 9%; dimethylacetamide equilibration time at 5 degrees C, 1 vs 30min; thawing at 60 degrees C for 10 vs 50 degrees C for 30sec. Spermatozoa viability (EtBr exclusion procedure - stress test), mobility (Accudenz (R) swim-down test) and subjective motility were assessed in fresh and frozen-thawed semen.3. The lower SWC (1.5 x 10(9) cells/ml) and the higher dimethylacetamide concentration (9%) had positive significant effects on the recovery rate of motile (22% vs 16%) and viable spermatozoa (39 vs 34%), respectively.4.Membrane (SYBR14-PI staining) and DNA integrity (comet assay) were assessed before and after freezing/thawing according to the optimised protocol.5. Recovery rates of spermatozoa with undamaged plasma membrane and DNA were 41% and 76%, respectively. The distribution of spermatozoa in classes of DNA damage was also analysed and discussed.6. It was concluded that pellet cryopreservation was a damaging process mainly for plasma membrane rather than nuclear DNA in chicken spermatozoa.
Chickens; DNA integrity; pellet cryopreservation; plasma membrane integrity; spermatozoa; Food Science; Animal Science and Zoology
Settore AGR/20 - Zoocolture
2017
Article (author)
File in questo prodotto:
File Dimensione Formato  
BPS_2017_comet_hubbard_cryopreservation.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 988.58 kB
Formato Adobe PDF
988.58 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/559215
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 5
  • ???jsp.display-item.citation.isi??? 5
social impact