BACKGROUD: It is estimated that about 3% of world population is affected by HCV virus. Most of affected male is during reproductive age. The sexual way of infection is doubtful even now. It is estimated that HCV RNA is presented in semen in 11% - 31.6% of cases. HCV RNA has been found in semen plasma and in not-spermatic cells, but not in sperm before and after swim-up. Aim of our study is to evaluate the HCV patients’ semen parameters compared to WHO 2010 semen parameters. MATERIALS AND METHODS: It is an observational study that include 58 patients following by our Fertility Center located in Luigi Sacco Hospital, in Milan, during 2010 to 2015. This patients’ group was affected by HCV chronic infection, nobody had hepatitis B, HIV or genital infections. All semen analysis was performed by the same biologist and in the same laboratory, after 3-5 days of sexual abstinence. RESULTS: Population’s study characteristics are described in the table 1. Patients 58 Age 43±6 Virus values 4810174 (range 1020-36091104) Way of infectious Unknown 58 (100%) TABLE 1 Table 2 shows results of comparison between patients HVC RNA positive and WHO fertile reference group. HCV male group presents median semen parameters significant lower than median semen parameters according to WHO 2010 values. About 50% of HCV patients have morphology’ and motility’s values below the fifth percentile of WHO reference values. Semen parameters HCV patients (n=58) Lower limit parameter WHO Population WHO Patients (%) Outside reference WHO parameter P value Volume (ml) 2,5 (0,5-6) 1,5 3,7 34,5 <0,05 Sperm concentration (10⁶/ml) 24,5 (0-123) 15 73 27,6 <0,05 Total sperm count (10⁶) 54,5 (0-297,5) 39 255 32,8 <0,05 Progressive Motility 34(0-60) 32 55 48,3 <0,05 Normal Morphology (%) 5 (1-27) 4 15 50 <0,05 TABLE 2 CONCLUSION: Our study provides evidence that all semen parameters of HVC-positive men are impaired comparing to WHO 2010 reference values and therefore a possible altered fertility in this group. Oxidative stress as a host response to HCV core protein and reactive oxidative species produced could damage sperm DNA and alter sperm quality. Concluding our Fertility center strongly suggests reproductive counselling in all couples with HCV male partner who desire a pregnancy.

Should HCV chronic infection impairer male fertility? / V. Savasi, E. Grossi, B. Parilla, M. Oneta, M. Musto, I. Cetin. ((Intervento presentato al convegno Congresso Nazionale SIMP tenutosi a Assisi nel 2015.

Should HCV chronic infection impairer male fertility?

V. Savasi
Primo
;
E. Grossi
Secondo
;
M. Musto
Penultimo
;
I. Cetin
Ultimo
2015

Abstract

BACKGROUD: It is estimated that about 3% of world population is affected by HCV virus. Most of affected male is during reproductive age. The sexual way of infection is doubtful even now. It is estimated that HCV RNA is presented in semen in 11% - 31.6% of cases. HCV RNA has been found in semen plasma and in not-spermatic cells, but not in sperm before and after swim-up. Aim of our study is to evaluate the HCV patients’ semen parameters compared to WHO 2010 semen parameters. MATERIALS AND METHODS: It is an observational study that include 58 patients following by our Fertility Center located in Luigi Sacco Hospital, in Milan, during 2010 to 2015. This patients’ group was affected by HCV chronic infection, nobody had hepatitis B, HIV or genital infections. All semen analysis was performed by the same biologist and in the same laboratory, after 3-5 days of sexual abstinence. RESULTS: Population’s study characteristics are described in the table 1. Patients 58 Age 43±6 Virus values 4810174 (range 1020-36091104) Way of infectious Unknown 58 (100%) TABLE 1 Table 2 shows results of comparison between patients HVC RNA positive and WHO fertile reference group. HCV male group presents median semen parameters significant lower than median semen parameters according to WHO 2010 values. About 50% of HCV patients have morphology’ and motility’s values below the fifth percentile of WHO reference values. Semen parameters HCV patients (n=58) Lower limit parameter WHO Population WHO Patients (%) Outside reference WHO parameter P value Volume (ml) 2,5 (0,5-6) 1,5 3,7 34,5 <0,05 Sperm concentration (10⁶/ml) 24,5 (0-123) 15 73 27,6 <0,05 Total sperm count (10⁶) 54,5 (0-297,5) 39 255 32,8 <0,05 Progressive Motility 34(0-60) 32 55 48,3 <0,05 Normal Morphology (%) 5 (1-27) 4 15 50 <0,05 TABLE 2 CONCLUSION: Our study provides evidence that all semen parameters of HVC-positive men are impaired comparing to WHO 2010 reference values and therefore a possible altered fertility in this group. Oxidative stress as a host response to HCV core protein and reactive oxidative species produced could damage sperm DNA and alter sperm quality. Concluding our Fertility center strongly suggests reproductive counselling in all couples with HCV male partner who desire a pregnancy.
Settore MED/40 - Ginecologia e Ostetricia
Should HCV chronic infection impairer male fertility? / V. Savasi, E. Grossi, B. Parilla, M. Oneta, M. Musto, I. Cetin. ((Intervento presentato al convegno Congresso Nazionale SIMP tenutosi a Assisi nel 2015.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/558203
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