A short course of anti-CD45RB leads to long-term islet allograft survival and donor-specific tolerance in approximately half of immunocompetent mice. We have previously demonstrated that anti-CD45RB antibodymediated tolerance requires B-cells for cardiac allograft survival. We therefore asked whether B-cells were also required for anti-CD45RB antibody-mediated survival of islets. Unexpectedly, we found that nearly 100% of islet allografts survive long term in B-cell-deficient mice. Similarly, B-cell depletion by anti-CD22/cal augmented anti-CD45RB-mediated tolerance when administered pretransplant, although it had no effect on tolerance induction when administered posttransplant. Our results demonstrate that the role of B-cells in promoting tolerance with anti-CD45RB is graft specific, promoting tolerance in cardiac grafts but resisting tolerance in islet transplantation. These findings may help elucidate the varied action of B-cells in promoting tolerance versus rejection.

B-Cell depletion improves islet allograft survival with anti-CD45RB / K.M. Lee, H. Yeh, G. Zhao, L. Wei, M. O'Connor, R.T. Stott, J. Soohoo, K. Dunussi, P. Fiorina, S. Deng, J.F. Markmann, J.I. Kim. - In: CELL TRANSPLANTATION. - ISSN 0963-6897. - 23:1(2014 Jun), pp. 51-58. [10.3727/096368912X658962]

B-Cell depletion improves islet allograft survival with anti-CD45RB

P. Fiorina;
2014-06

Abstract

A short course of anti-CD45RB leads to long-term islet allograft survival and donor-specific tolerance in approximately half of immunocompetent mice. We have previously demonstrated that anti-CD45RB antibodymediated tolerance requires B-cells for cardiac allograft survival. We therefore asked whether B-cells were also required for anti-CD45RB antibody-mediated survival of islets. Unexpectedly, we found that nearly 100% of islet allografts survive long term in B-cell-deficient mice. Similarly, B-cell depletion by anti-CD22/cal augmented anti-CD45RB-mediated tolerance when administered pretransplant, although it had no effect on tolerance induction when administered posttransplant. Our results demonstrate that the role of B-cells in promoting tolerance with anti-CD45RB is graft specific, promoting tolerance in cardiac grafts but resisting tolerance in islet transplantation. These findings may help elucidate the varied action of B-cells in promoting tolerance versus rejection.
Anti-CD22; Anti-CD45RB; B-cells; Islet transplantation; Tolerance; Allografts; Animals; B-Lymphocytes; Diabetes Mellitus, Experimental; Disease Models, Animal; Graft Survival; Islets of Langerhans Transplantation; Leukocyte Common Antigens; Male; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Transplantation Tolerance; Biomedical Engineering; Cell Biology; Transplantation
Settore MED/13 - Endocrinologia
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/556991
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