Epidermolysis bullosa acquisita is a rare autoimmune bullous disease characterized by the presence of circulating antibodies directed against the collagen type VII. Diagnosis is generally based on clinical history, clinical features, histology, direct and indirect immunofluorescence, immunoblotting and ELISA. Our study aims to determine the validity of the Biochip immunofluorescence microscopy for the serological diagnosis of epidermolysis bullosa acquisita. Six patients with epidermolysis bullosa acquisita and presence of antibodies against type VII collagen confirmed by ELISA were included in the study. Subsequently, all sera of patients were analyzed using Biochip. Antibodies anti-collagen type VII were detected in all sera by means of the Biochip technology. Thus, Biochip shows a good correlation with ELISA and seems to be an appropriate method for the diagnosis of epidermolysis bullosa acquisita. It is an easy, fast and standardized method which could facilitate the diagnosis of this autoimmune bullous disease. We suggest that it could be used as an initial screening test to identify patients with epidermolysis bullosa acquisita.
|Titolo:||The use of Biochip immunofluorescence microscopy for the serological diagnosis of epidermolysis bullosa acquisita|
|Parole Chiave:||Biochip technology; ELISA; Epidermolysis bullosa acquisita; Serological diagnosis; Autoantibodies; Cell Line; Collagen Type VII; Enzyme-Linked Immunosorbent Assay; Epidermolysis Bullosa Acquisita; Female; HEK293 Cells; Humans; Male; Pilot Projects; Protein Array Analysis; Microscopy, Fluorescence; 2708|
|Settore Scientifico Disciplinare:||Settore MED/35 - Malattie Cutanee e Veneree|
|Data di pubblicazione:||2016|
|Digital Object Identifier (DOI):||10.1007/s00403-016-1632-0|
|Appare nelle tipologie:||01 - Articolo su periodico|