The recently determined crystal structures of the sequence-specific transcription factor NF-Y have illuminated the structural mechanism underlying transcription at the CCAAT box. NF-Y is a trimeric protein complex composed by the NF-YA, NF-YB, and NF-YC subunits. NF-YB and NF-YC contain a histone-like domain and assemble on a head-to-tail fashion to form a dimer, which provides the structural scaffold for the DNA sugar-phosphate backbone binding (mimicking the nucleosome H2A/H2BâDNA assembly) and for the interaction with NF-YA. The NF-YA subunit hosts two structurally extended Î±-helices; one is involved in NF-YB/NF-YC binding and the other inserts deeply into the DNA minor groove, providing exquisite sequence-specificity for recognition and binding of the CCAAT box. The analysis of these structural data is expected to serve as a powerful guide for future experiments aimed at understanding the role of post-translational modification at NF-Y regulation sites and to unravel the three-dimensional architecture of higher order complexes formed between NF-Y and other transcription factors that act synergistically for transcription activation. Moreover, these structures represent an excellent starting point to challenge the formation of a stable hybrid nucleosome between NF-Y and core histone proteins, and to rationalize the fine molecular details associated with the wide combinatorial association of plant NF-Y subunits.
Structural determinants for NF-Y/DNA interaction at the CCAAT box / V. Nardone, A. Chaves-Sanjuan, M. Nardini. - In: BIOCHIMICA ET BIOPHYSICA ACTA. GENE REGULATORY MECHANISMS. - ISSN 1874-9399. - 1860:5(2017), pp. 571-580.
|Titolo:||Structural determinants for NF-Y/DNA interaction at the CCAAT box|
NARDINI, MARCO (Corresponding)
|Parole Chiave:||Transcription factor; X-ray crystallography; NF-Y; Histones; DNA-binding|
|Settore Scientifico Disciplinare:||Settore BIO/10 - Biochimica|
|Data di pubblicazione:||2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.bbagrm.2016.09.006|
|Appare nelle tipologie:||01 - Articolo su periodico|