OBJECTIVE: Maternal obesity is characterized by systemic low-grade inflammation and oxidative stress (OxS) with the contribution of fetal sex dimorphism. We recently described increased mitochondrial content (mtDNA) in placentas of obese pregnancies. Here, we quantify mtDNA and hepcidin as indexes of OxS and systemic inflammation in the obese maternal circulation. METHODS: Forty-one pregnant women were enrolled at elective cesarean section: 16 were normal weight (NW) and 25 were obese (OB). Obese women were further classified according to the presence/absence of maternal gestational diabetes mellitus (GDM); [OB/GDM(-)]: n = 15, [OB/GDM(+)]: n = 10. mtDNA and hepcidin were evaluated in blood (real-time PCR) and plasma (ELISA). RESULTS: mtDNA and hepcidin levels were significantly increased in OB/GDM(-) versus NW, significantly correlating with pregestational BMI. Male/female (M/F) ratio was equal in study groups, and overall F-carrying pregnancies showed significantly higher mtDNA and hepcidin levels than M-carrying pregnancies both in obese and normal weight mothers. CONCLUSIONS: Our results indicate a potential compensatory mechanism to increased obesity-related OxS and inflammation, indicated by the higher hepcidin levels found in obese mothers. Increased placental mitochondrial biogenesis, due to lipotoxic environment, may account for the greater mtDNA amount released in maternal circulation. This increase is namely related to F-carrying pregnancies, suggesting a gender-specific placental response.
Mitochondrial Content and Hepcidin are Increased in Obese Pregnant Mothers / G.M. Anelli, M. Cardellicchio, C. Novielli, P. Antonazzo, M.I. Mazzocco, I. Cetin, C. Mandò. - In: THE JOURNAL OF MATERNAL-FETAL & NEONATAL MEDICINE. - ISSN 1476-7058. - 31:18(2018 May 21), pp. 2388-2395.
|Titolo:||Mitochondrial Content and Hepcidin are Increased in Obese Pregnant Mothers|
ANELLI, GAIA MARIA [Writing – Original Draft Preparation]
|Parole Chiave:||gestational diabetes mellitus; hepcidin; maternal obesity; mitochondrial DNA; sexual dimorphism|
|Settore Scientifico Disciplinare:||Settore MED/40 - Ginecologia e Ostetricia|
Settore BIO/11 - Biologia Molecolare
|Data di pubblicazione:||21-mag-2018|
|Data ahead of print / Data di stampa:||4-lug-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1080/14767058.2017.1344209|
|Appare nelle tipologie:||01 - Articolo su periodico|