It is well established that epigenetic modulation of genome accessibility in chromatin occurs during biological processes. Here we describe a method based on restriction enzymes and next-generation sequencing for identifying accessible DNA elements using a small amount of starting material, and use it to examine myeloid differentiation of primary human CD34+ cells. The accessibility of several classes of cis-regulatory elements was a predictive marker of in vivo DNA binding by transcription factors, and was associated with distinct patterns of histone posttranslational modifications. We also mapped large chromosomal domains with differential accessibility in progenitors and maturing cells. Accessibility became restricted during differentiation, correlating with a decreased number of expressed genes and loss of regulatory potential. Our data suggest that a permissive chromatin structure in multipotent cells is progressively and selectively closed during differentiation, and illustrate the use of our method for the identification of functional cis-regulatory elements.

NA-Seq: a discovery tool for the analysis of chromatin structure and dynamics during differentiation / G. Gargiulo, S. Levy, G. Bucci, M. Romanenghi, L. Fornasari, K. Y. Beeson, S. M. Goldberg, M. Cesaroni, M. Ballarini, F. Santoro, N. Bezman, G. Frigè, P. D. Gregory, M. C. Holmes, R. L. Strausberg, P. G. Pelicci, F. D. Urnov, S. Minucci. - In: DEVELOPMENTAL CELL. - ISSN 1534-5807. - 16:3(2009), pp. 466-481.

NA-Seq: a discovery tool for the analysis of chromatin structure and dynamics during differentiation

G. Gargiulo
Primo
;
G. Bucci;G. Frigè;P. G. Pelicci;S. Minucci
Ultimo
2009

Abstract

It is well established that epigenetic modulation of genome accessibility in chromatin occurs during biological processes. Here we describe a method based on restriction enzymes and next-generation sequencing for identifying accessible DNA elements using a small amount of starting material, and use it to examine myeloid differentiation of primary human CD34+ cells. The accessibility of several classes of cis-regulatory elements was a predictive marker of in vivo DNA binding by transcription factors, and was associated with distinct patterns of histone posttranslational modifications. We also mapped large chromosomal domains with differential accessibility in progenitors and maturing cells. Accessibility became restricted during differentiation, correlating with a decreased number of expressed genes and loss of regulatory potential. Our data suggest that a permissive chromatin structure in multipotent cells is progressively and selectively closed during differentiation, and illustrate the use of our method for the identification of functional cis-regulatory elements.
DEVBIO; DNA
Settore MED/04 - Patologia Generale
Article (author)
File in questo prodotto:
Non ci sono file associati a questo prodotto.
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/55110
Citazioni
  • ???jsp.display-item.citation.pmc??? 27
  • Scopus 49
  • ???jsp.display-item.citation.isi??? 45
social impact