Progesterone Receptor Membrane Component-1 (PGRMC1) is a highly conserved multifunctional protein that is found in numerous systems, including reproductive system. Interestingly, PGRMC1 is expressed at several intracellular locations, including the nucleolus. The aim of this study is to investigate the functional relationship between PGRMC1 and nucleolus. Immunofluorescence experiments confirmed PGRMC1's nucleolar localization in cultured bovine granulosa cells (bGC) and oocytes. Additional experiments conducted on bGC revealed that PGRMC1 co-localizes with nucleolin (NCL), a major nucleolar protein. Furthermore, small interfering RNA (RNAi) mediated gene-silencing experiments showed that when PGRMC1 expression was depleted, NCL translocated from the nucleolus to the nucleoplasm. Similarly, oxidative stress induced by hydrogen peroxide (H2O2) treatment, reduced PGRMC1 immunofluorescent signal in the nucleolus and increased NCL nucleoplasmic signal, when compared to non-treated cells. Although PGRMC1 influenced NCL localization, a direct interaction between these two proteins was not detected using in situ proximity ligation assay. This suggests the involvement of additional molecules in mediating the co-localization of PGRMC1 and nucleolin. Since nucleolin translocates into the nucleoplasm in response to various cellular stressors, PGRMC1's ability to regulate its localization within the nucleolus is likely an important component of mechanism by which cells response to stress. This concept is consistent with PGRMC1's well-described ability to promote ovarian cell survival and provides a rationale for future studies on PGRMC1, NCL and the molecular mechanism by which these two proteins protect against the adverse effect of cellular stressors, including oxidative stress.

PGRMC1 localization and putative function in the nucleolus of bovine granulosa cells and oocytes / L. Terzaghi, A.M. Luciano, P.C. Dall Acqua, S. Modina, J.J. Peluso, V. Lodde. - In: REPRODUCTION. - ISSN 1470-1626. - 155:3(2018 Mar), pp. 273-282. [10.1530/REP-17-0534]

PGRMC1 localization and putative function in the nucleolus of bovine granulosa cells and oocytes

L. Terzaghi
Primo
;
A.M. Luciano
Secondo
;
S. Modina;V. Lodde
Ultimo
2018

Abstract

Progesterone Receptor Membrane Component-1 (PGRMC1) is a highly conserved multifunctional protein that is found in numerous systems, including reproductive system. Interestingly, PGRMC1 is expressed at several intracellular locations, including the nucleolus. The aim of this study is to investigate the functional relationship between PGRMC1 and nucleolus. Immunofluorescence experiments confirmed PGRMC1's nucleolar localization in cultured bovine granulosa cells (bGC) and oocytes. Additional experiments conducted on bGC revealed that PGRMC1 co-localizes with nucleolin (NCL), a major nucleolar protein. Furthermore, small interfering RNA (RNAi) mediated gene-silencing experiments showed that when PGRMC1 expression was depleted, NCL translocated from the nucleolus to the nucleoplasm. Similarly, oxidative stress induced by hydrogen peroxide (H2O2) treatment, reduced PGRMC1 immunofluorescent signal in the nucleolus and increased NCL nucleoplasmic signal, when compared to non-treated cells. Although PGRMC1 influenced NCL localization, a direct interaction between these two proteins was not detected using in situ proximity ligation assay. This suggests the involvement of additional molecules in mediating the co-localization of PGRMC1 and nucleolin. Since nucleolin translocates into the nucleoplasm in response to various cellular stressors, PGRMC1's ability to regulate its localization within the nucleolus is likely an important component of mechanism by which cells response to stress. This concept is consistent with PGRMC1's well-described ability to promote ovarian cell survival and provides a rationale for future studies on PGRMC1, NCL and the molecular mechanism by which these two proteins protect against the adverse effect of cellular stressors, including oxidative stress.
English
Settore VET/01 - Anatomia degli Animali Domestici
Settore VET/02 - Fisiologia Veterinaria
Settore BIO/06 - Anatomia Comparata e Citologia
Settore BIO/09 - Fisiologia
Settore BIO/11 - Biologia Molecolare
Settore BIO/17 - Istologia
Articolo
Esperti anonimi
Ricerca di base
Pubblicazione scientifica
   Role of Progesterone Receptor Membrane Component-1 in oogenesis and mammalian fertility
   PRO-OVUM
   EUROPEAN COMMISSION
   FP7
   303640
mar-2018
16-gen-2018
BioScientifica
155
3
273
282
10
Pubblicato
Periodico con rilevanza internazionale
crossref
pubmed
Aderisco
info:eu-repo/semantics/article
PGRMC1 localization and putative function in the nucleolus of bovine granulosa cells and oocytes / L. Terzaghi, A.M. Luciano, P.C. Dall Acqua, S. Modina, J.J. Peluso, V. Lodde. - In: REPRODUCTION. - ISSN 1470-1626. - 155:3(2018 Mar), pp. 273-282. [10.1530/REP-17-0534]
partially_open
Prodotti della ricerca::01 - Articolo su periodico
6
262
Article (author)
Periodico con Impact Factor
L. Terzaghi, A.M. Luciano, P.C. Dall Acqua, S. Modina, J.J. Peluso, V. Lodde
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/550231
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