Keratinocytes (KCs) and Langerhans cells (LCs) contribute to create the epidermal barrier. To form a functional epidermis, KCs express filaggrin and Toll-like Receptors (TLRs). LCs are the first line of epidermal defence and can be activated by interleukin (IL)-17 and Tumor Necrosis Factor (TNF)-alpha. In psoriasis, an alteration of TLR expression, a defective expression of filaggrin, and LC activation occur. In organotypic cultures of human skin we investigated the interplay between IL-17 and TNF-alpha on i) expression of filaggrin, TLR2, 7 and 9, and Nuclear Factor (NF)-kB localization by immunofluorescence and ii) LC ultrastructural features by transmission electron microscopy. Normal human skin was obtained after aesthetic surgery (n=7), overnight incubated in a Transwell system, and exposed to TNF-alpha and/or IL-17 for 24 (T24), 48 (T48), and 72 (T72) hours. Cytokines always influenced the expression of filaggrin. TNF-alpha alone activated LCs only starting from T48. TLR2 and TLR7 expressions were affected at T24 by IL-17 and the combination of cytokines, but not by TNF-alpha. TLR9-positive cells were detectable in the granular layer after cytokine exposure. A nuclear localization of NF-kB was always observed after cytokine incubation. In conclusion, each cytokine possess an intrinsic activity on the different components of the epidermal barrier.
Epidermal barrier reaction to an in vitro psoriatic microenvironment / E. Donetti, L. Cornaghi, F. Arnaboldi, F. Ricceri, L. Pescitelli, M. Maiocchi, F. Carriero, F. Baruffaldi Preis, F. Prignano. - In: EXPERIMENTAL CELL RESEARCH. - ISSN 0014-4827. - 15:2(2017 Nov 15), pp. 180-188.
|Titolo:||Epidermal barrier reaction to an in vitro psoriatic microenvironment|
DONETTI, ELENA BIANCA (Primo) [Writing – Original Draft Preparation] (Corresponding)
CORNAGHI, LAURA BRIGIDA (Secondo) [Investigation]
ARNABOLDI, FRANCESCA [Investigation]
|Parole Chiave:||TLRs; transmission electron microscopy; immunofluorescence; innate immunity; nuclear factor kappa B; langerhans cells|
|Settore Scientifico Disciplinare:||Settore BIO/16 - Anatomia Umana|
|Data di pubblicazione:||15-nov-2017|
|Data ahead of print / Data di stampa:||set-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.yexcr.2017.09.004|
|Appare nelle tipologie:||01 - Articolo su periodico|