The performance of a rapid immunochromatographic test for the detection of Dirofilaria immitis antigens (Speed Diro (TM); BVT-Virbac, France) was assessed in 49 experimentally infected dogs and in 244 naturally infected animals; 142 dogs and 102 cats. In experimentally infected dogs, Speed Diro (TM) showed a sensitivity of 90.9% in dogs infected with one adult female worm and 100% in dogs infected with more than one female worm. Specificity was 100%. For naturally infected dogs, the Knott test and PetChek(A (R)) HTWM PF served as reference methods for microfilaremia and antigenemia, respectively. All microfilaemic dogs (55/142) were positive with Speed Diro (TM). Importantly, none of the 21 dogs infected with D. repens were positive. The results of Speed Diro (TM) for the detection of antigenemia were compared with two in-house tests, SNAP(A (R)) HTWM and Witness(A (R)) Dirofilaria, and all three tests were 100% specific and sensitive in comparison to PetChek(A (R)) HTWM PF. For the evaluation of feline samples, 102 cats were examined by echocardiography. Sera from 87 heartworm-infected cats were tested by Speed Diro (TM) and SNAP(A (R)) HTWM. The results of Speed Diro (TM) were equivalent to SNAP(A (R)) HTWM, with a sensitivity of 98.9% and a specificity of 100%.
Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats / M. Genchi, C. Mangia, N. Ferrari, S. Loukeri. - In: PARASITOLOGY RESEARCH. - ISSN 0932-0113. - 117:1(2018 Jan), pp. 31-34.
|Titolo:||Evaluation of a rapid immunochromatographic test for the detection of low burden Dirofilaria immitis (heartworm) in dogs and cats|
GENCHI, MARCO (Primo) [Writing – Review & Editing]
FERRARI, NICOLA [Methodology]
|Parole Chiave:||Dirofilaria immitis; Heartworm; dogs; cats; antigen test; Speed Diro (TM); sensitivity; specificity|
|Settore Scientifico Disciplinare:||Settore VET/06 - Parassitologia e Malattie Parassitarie degli Animali|
|Data di pubblicazione:||gen-2018|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00436-017-5709-2|
|Appare nelle tipologie:||01 - Articolo su periodico|