Background: Antimicrobial peptides (AMPs) are an ancient group of defense molecules. AMPs are widely distributed in nature (being present in mammals, birds, amphibians, insects, plants, and microorganisms). They display bactericidal as well as immunomodulatory properties. The aim of this study was to investigate the antimicrobial and anti-inflammatory activities of a combination of two AMPs (temporin B and the royal jellein I) against Staphylococcus epidermidis.Results: The temporin B (TB-KK) and the royal jelleins I, II, III chemically modified at the C terminal (RJI-C, RJII-C, RJIII-C), were tested for their activity against 10 different Staphylococcus epidermidis strains, alone and in combination. Of the three royal jelleins, RJI-C showed the highest activity. Moreover, the combination of RJI-C and TB-KK (MIX) displayed synergistic activity. In vitro, the MIX displayed low hemolytic activity, no NO2- production and the ability to curb the synthesis of the pro-inflammatory cytokines TNF-α and IFN-γ to the same extent as acetylsalicylic acid. In vivo, the MIX sterilized mice infected with Staphylococcus epidermidis in eleven days and inhibited the expression of genes encoding the prostaglandin-endoperoxide synthase 2 (COX-2) and CD64, two important parameters of inflammation.Conclusion: The study shows that the MIX - a combination of two naturally occurring peptides - displays both antimicrobial and anti-inflammatory activities.

New perspectives for natural antimicrobial peptides : Application as antinflammatory drugs in a murine model / C. Rosanna, D.C. Francesco, N. Nunzia, M. Rosa C., I. Marco, F. Andrea, A. Romanelli, A. Concetta, B. Giuseppe, C. Federico. - In: BMC IMMUNOLOGY. - ISSN 1471-2172. - 13:1(2012 Nov 17). [10.1186/1471-2172-13-61]

New perspectives for natural antimicrobial peptides : Application as antinflammatory drugs in a murine model

A. Romanelli
Membro del Collaboration Group
;
2012

Abstract

Background: Antimicrobial peptides (AMPs) are an ancient group of defense molecules. AMPs are widely distributed in nature (being present in mammals, birds, amphibians, insects, plants, and microorganisms). They display bactericidal as well as immunomodulatory properties. The aim of this study was to investigate the antimicrobial and anti-inflammatory activities of a combination of two AMPs (temporin B and the royal jellein I) against Staphylococcus epidermidis.Results: The temporin B (TB-KK) and the royal jelleins I, II, III chemically modified at the C terminal (RJI-C, RJII-C, RJIII-C), were tested for their activity against 10 different Staphylococcus epidermidis strains, alone and in combination. Of the three royal jelleins, RJI-C showed the highest activity. Moreover, the combination of RJI-C and TB-KK (MIX) displayed synergistic activity. In vitro, the MIX displayed low hemolytic activity, no NO2- production and the ability to curb the synthesis of the pro-inflammatory cytokines TNF-α and IFN-γ to the same extent as acetylsalicylic acid. In vivo, the MIX sterilized mice infected with Staphylococcus epidermidis in eleven days and inhibited the expression of genes encoding the prostaglandin-endoperoxide synthase 2 (COX-2) and CD64, two important parameters of inflammation.Conclusion: The study shows that the MIX - a combination of two naturally occurring peptides - displays both antimicrobial and anti-inflammatory activities.
Administration, Intravenous; Amino Acid Sequence; Animals; Anti-Bacterial Agents; Anti-Infective Agents; Anti-Inflammatory Agents; Antimicrobial Cationic Peptides; Bacterial Load; Cell Death; Disease Models, Animal; Flow Cytometry; Gene Expression Regulation; Gentamicins; Hemolysis; Lipopolysaccharides; Macrophages; Mice; Microbial Sensitivity Tests; Molecular Sequence Data; Nitrates; RNA, Messenger; Staphylococcal Infections; Staphylococcus epidermidis; Immunology
Settore CHIM/03 - Chimica Generale e Inorganica
17-nov-2012
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/547547
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