The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.
Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery / C. Avitabile, A. Accardo, P. Ringhieri, G. Morelli, M. Saviano, G. Montagner, E. Fabbri, E. Gallerani, R. Gambari, A. Romanelli. - In: BIOCONJUGATE CHEMISTRY. - ISSN 1043-1802. - 26:8(2015), pp. 1533-1541. [10.1021/acs.bioconjchem.5b00156]
Incorporation of Naked Peptide Nucleic Acids into Liposomes Leads to Fast and Efficient Delivery
A. Romanelli
Ultimo
2015
Abstract
The delivery of peptide nucleic acids (PNAs) to cells is a very challenging task. We report here that a liposomal formulation composed of egg PC/cholesterol/DSPE-PEG2000 can be loaded, according to different encapsulation techniques, with PNA or fluorescent PNA oligomers. PNA loaded liposomes efficiently and quickly promote the uptake of a PNA targeting the microRNA miR-210 in human erythroleukemic K562 cells. By using this innovative delivery system for PNA, down-regulation of miR-210 is achieved at a low PNA concentration.
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