MicroRNAs (miRNAs) are highly conserved elements in mammals, and exert key regulatory functions. Growing evidence shows that miRNAs can interact with another class of non-coding RNAs, so-called transcribed ultraconserved regions (T-UCRs), which take part in transcriptional, post-transcriptional and epigenetic regulation processes. We report here the interaction of miRNAs and T-UCRs as a network modulating the availability of these non-coding RNAs in bladder cancer cells. In our cell system, antagomiR-596 increased the expression of T-UCR 201+. Moreover, T-UCR 8+ silencing increased miR-596 expression, which in turn reduced total T-UCR 283+, showing that the perturbation of one element in this network changes the expression of other interactors. In addition, we identify the polycomb protein Yin Yang 1 (YY1) as mediator of binding between miR-596 and T-UCR 8+. These new findings describe for the first time a network between T-UCRs, miRNAs and YY1 protein, highlighting the existence of an additional layer of gene expression regulation.
New cross-talk layer between ultraconserved non-coding RNAs, microRNAs and polycomb protein YY1 in bladder cancer / S. Terreri, M. Durso, V. Colonna, A. Romanelli, D. Terracciano, M. Ferro, S. Perdonà, L. Castaldo, F. Febbraio, F. de Nigris, A. Cimmino. - In: GENES. - ISSN 2073-4425. - 7:12(2016).
|Titolo:||New cross-talk layer between ultraconserved non-coding RNAs, microRNAs and polycomb protein YY1 in bladder cancer|
ROMANELLI, ALESSANDRA [Membro del Collaboration Group]
|Parole Chiave:||bladder cancer; interaction network; MicroRNAs; transcribed ultraconserved regions; genetics; genetics (clinical)|
|Settore Scientifico Disciplinare:||Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica|
Settore CHIM/03 - Chimica Generale e Inorganica
|Data di pubblicazione:||2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/genes7120127|
|Appare nelle tipologie:||01 - Articolo su periodico|