Over recent years it has emerged how certain no crop-species can be employed in phytoremediating contaminated soils or preventing herbicide pollution; in this contest Festuca arundinacea was investigated. Shoots of Festuca were submitted to fast protein liquid chromatography in order to identify their glutathione S-transferases (GST; EC 22.214.171.124), by a combination of anionic, affinity and RP-HPLC chromatography. The chromatographic procedure revealed satisfactory yield and four GSTs were identified: they were named FaGST I, FaGST II, FaGST III and FaGST IV. Among these, significant differences were observed in the chromatographic behaviours, structure, activity toward a ‘‘model’’ substrate, 1-chloro-2,4-dinitrobenzene, and responsiveness to the herbicide safener benoxacor. FaGST I showed the highest activity toward the above substrate, and this activity was up-regulated by the herbicide safener. Therefore, FaGST I was purified till homogeneity and was determined to be an heterodimer consisting of two subunits of 28.0 and 27.2 kDa. Each subunit of FaGST I was further characterized by means of LC–ESI–MS/MS and immunoblotting analysis, which revealed that both the subunits belong to the tau subclass.
|Titolo:||Glutathione S-transferases in Festuca arundinacea : Identification, characterization and inducibility by safener benoxacor|
|Autori interni:||ESPEN, LUCA (Ultimo)|
|Parole Chiave:||GSTs ; Festuca arundinacea ; herbicide ; herbicide safener|
|Settore Scientifico Disciplinare:||Settore AGR/13 - Chimica Agraria|
|Data di pubblicazione:||20-lug-2007|
|Digital Object Identifier (DOI):||10.1016/j.phytochem.2007.05.041|
|Appare nelle tipologie:||01 - Articolo su periodico|