Plants react to the attack of pathogen microorganisms by mounting appropriate and efficient downstream defense responses often involving a form of localized cell death called hypersensitive response (HR).Here we describe an innovative and noninvasive protocol based on in vivo bioimaging technique coupled with utilization of genetically encoded fluorescent sensors that allows to monitor and analyze intracellular calcium (Ca2+) dynamics and changes of the glutathione redox status taking place in plant organs during plant interaction with the HR-inducing bacteria Pseudomonas syringae (PstAvrB).

In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB) / F.G. Doccula, L. Luoni, S. Behera, M.C. Bonza, A. Costa (METHODS IN MOLECULAR BIOLOGY). - In: Plant Programmed Cell Death / Doccula, Fabrizio Gandolfo ; [a cura di] De Gara L., Locato V.. - Prima edizione. - [s.l] : Springer, 2018 Jan. - ISBN 978-1-4939-7667-6. - pp. 125-141 [10.1007/978-1-4939-7668-3_12]

In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB)

F.G. Doccula
Membro del Collaboration Group
;
L. Luoni
Membro del Collaboration Group
;
S. Behera
Membro del Collaboration Group
;
M.C. Bonza
Membro del Collaboration Group
;
A. Costa
Writing – Review & Editing
2018-01

Abstract

Plants react to the attack of pathogen microorganisms by mounting appropriate and efficient downstream defense responses often involving a form of localized cell death called hypersensitive response (HR).Here we describe an innovative and noninvasive protocol based on in vivo bioimaging technique coupled with utilization of genetically encoded fluorescent sensors that allows to monitor and analyze intracellular calcium (Ca2+) dynamics and changes of the glutathione redox status taking place in plant organs during plant interaction with the HR-inducing bacteria Pseudomonas syringae (PstAvrB).
Cameleon calcium sensor; GRX-roGFP2 sensor; Hypersensitive response; In vivo imaging
Settore BIO/04 - Fisiologia Vegetale
https://link.springer.com/protocol/10.1007/978-1-4939-7668-3_12
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/544666
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