AIM: This in vitro and in vivo study reports on silk fibroin (SF) scaffold, functionalized for in situ delivery of GABA and/or allopregnanolone (ALLO), as biomaterial for potential application in tissue engineering and nerve regeneration. MATERIALS & METHODS: We evaluated the feasibility to design 2D scaffolds (films) made of regenerated Bombyx mori SF, functionalized with GABA and/or ALLO to enhance in vitro biological functions, health, survival and growth of Schwann cells and sensitive neurons of the dorsal root ganglia. RESULTS & CONCLUSION: Our 2D-SF film showed an efficient loading and controllable release of drugs promoting nerve regeneration. SF functionalized film may be helpful for the development of bioengineered conduits and, in principle, have great potential for long-gap nerve injury repair.

In vitro and in vivo evaluation of silk fibroin functionalized with GABA and allopregnanolone for Schwann cell and neuron survival / C.G.M. Gennari, F. Cilurzo, N. Mitro, D. Caruso, P. Minghetti, V. Magnaghi. - In: REGENERATIVE MEDICINE. - ISSN 1746-0751. - 13:2(2018 Mar), pp. 141-157. [10.2217/rme-2017-0102]

In vitro and in vivo evaluation of silk fibroin functionalized with GABA and allopregnanolone for Schwann cell and neuron survival

C.G.M. Gennari
Primo
;
F. Cilurzo
Secondo
;
N. Mitro;D. Caruso;P. Minghetti
Penultimo
;
V. Magnaghi
Ultimo
2018-03

Abstract

AIM: This in vitro and in vivo study reports on silk fibroin (SF) scaffold, functionalized for in situ delivery of GABA and/or allopregnanolone (ALLO), as biomaterial for potential application in tissue engineering and nerve regeneration. MATERIALS & METHODS: We evaluated the feasibility to design 2D scaffolds (films) made of regenerated Bombyx mori SF, functionalized with GABA and/or ALLO to enhance in vitro biological functions, health, survival and growth of Schwann cells and sensitive neurons of the dorsal root ganglia. RESULTS & CONCLUSION: Our 2D-SF film showed an efficient loading and controllable release of drugs promoting nerve regeneration. SF functionalized film may be helpful for the development of bioengineered conduits and, in principle, have great potential for long-gap nerve injury repair.
2D scaffold; Schwann cell; fibroin; neuroactive steroid; peripheral nervous system
Settore BIO/09 - Fisiologia
Settore BIO/10 - Biochimica
Settore CHIM/09 - Farmaceutico Tecnologico Applicativo
21-nov-2017
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/543927
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