The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the ability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, such as sheep, goats, swine, cats, dogs, rabbits, mink, horses, turkeys, ferrets, monkeys, hamsters, rats, mice, and chickens. In this report, the feasibility to use BoHV-4 based vector in chicken was investigated. Although BoHV-4 was able to replicate, leading to a cytopathic effect in a chicken cell line and infect the chorion allantoic membrane of embryonated eggs, however it was not pathogenic even when a large dose of virus was injected into the chicken. An immune response could be produced against heterologous antigen delivered by a recombinant BoHV-4. These data suggest the feasibility of using BoHV-4 based vector for vaccination purposes in chickens.

Assessment of bovine herpesvirus 4 based vector in chicken / G. Donofrio, G. Manarolla, L. Ravanetti, G. Sironi, S. Cavirani, C.S. Cabassi, C.F. Flammini, T.Rampin. - In: JOURNAL OF VIROLOGICAL METHODS. - ISSN 0166-0934. - 148:1-2(2008 Mar), pp. 303-306.

Assessment of bovine herpesvirus 4 based vector in chicken

G. Manarolla
Secondo
;
G. Sironi;T.Rampin
Ultimo
2008-03

Abstract

The biological characteristics of BoHV-4 make it a good candidate as a gene delivery vector for vaccination purposes. These characteristics include little or no pathogenicity, unlikely oncogenicity, the ability to accommodate large amounts of foreign genetic material, the ability to infect several cell types from different animal species, such as sheep, goats, swine, cats, dogs, rabbits, mink, horses, turkeys, ferrets, monkeys, hamsters, rats, mice, and chickens. In this report, the feasibility to use BoHV-4 based vector in chicken was investigated. Although BoHV-4 was able to replicate, leading to a cytopathic effect in a chicken cell line and infect the chorion allantoic membrane of embryonated eggs, however it was not pathogenic even when a large dose of virus was injected into the chicken. An immune response could be produced against heterologous antigen delivered by a recombinant BoHV-4. These data suggest the feasibility of using BoHV-4 based vector for vaccination purposes in chickens.
Bovine herpesvirus 4 ; Chicken ; Vaccine vector
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
Settore VET/05 - Malattie Infettive degli Animali Domestici
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/54288
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