In recent decades, tissue engineering strategies have been proposed for the treatment of musculoskeletal diseases and bone fractures to overcome the limitations of the traditional surgical approaches based on allografts and autografts. In this work we report the development of a composite porous poly(dl-lactide-co-glycolide) scaffold suitable for bone regeneration. Scaffolds were produced by thermal sintering of porous microparticles. Next, in order to improve cell adhesion to the scaffold and subsequent proliferation, the scaffolds were coated with the osteoconductive biopolymers chitosan and sodium alginate, in a process that exploited electrostatic interactions between the positively charged biopolymers and the negatively charged PLGA scaffold. The resulting scaffolds were characterized in terms of porosity, degradation rate, mechanical properties, biocompatibility and suitability for bone regeneration. They were found to have an overall porosity of ∼85% and a degradation half time of ∼2 weeks, considered suitable to support de novo bone matrix deposition from mesenchymal stem cells. Histology confirmed the ability of the scaffold to sustain adipose-derived mesenchymal stem cell adhesion, infiltration, proliferation and osteo-differentiation. Histological staining of calcium and microanalysis confirmed the presence of calcium phosphate in the scaffold sections.
Biodegradable composite porous poly(dl-lactide-co-glycolide) scaffold supports mesenchymal stem cell differentiation and calcium phosphate deposition / S. Casagrande, R. Tiribuzi, E. Cassetti, F. Selmin, G.L. Gervasi, L. Barberini, M. Freddolini, M. Ricci, A. Schoubben, G.G. Cerulli, P. Blasi. - In: ARTIFICIAL CELLS, NANOMEDICINE, AND BIOTECHNOLOGY. - ISSN 2169-1401. - 46:suppl. 1(2018 Oct), pp. S219-S229.
|Titolo:||Biodegradable composite porous poly(dl-lactide-co-glycolide) scaffold supports mesenchymal stem cell differentiation and calcium phosphate deposition|
|Parole Chiave:||microparticle sintering; chitosan; alginate; bone tissue engineering; adipose-derived mesenchymal stem cells|
|Settore Scientifico Disciplinare:||Settore CHIM/09 - Farmaceutico Tecnologico Applicativo|
|Data di pubblicazione:||ott-2018|
|Data ahead of print / Data di stampa:||21-dic-2017|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1080/21691401.2017.1417866|
|Appare nelle tipologie:||01 - Articolo su periodico|
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